Evidence map›Paper›PMID 28646859›Full record

ArticleBMC veterinary research2017

Comparison of different NAT assays for the detection of microorganisms belonging to the class Mollicutes.

O Vega-Orellana, J B Poveda, R S Rosales, J M Bradbury, C G Poveda, L E Mederos-Iriarte, M M Tavío, A S Ramírez

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. MinisculeSustainable microbiology · 2024
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

O Vega-OrellanaUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain.
J B PovedaUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain.
R S RosalesUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain. ruben.rosales@ulpgc.es.
J M BradburyUniversity of Liverpool, School of Veterinary Science, Leahurst Campus, Neston, CH64 7TE, UK.
C G PovedaUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain.
L E Mederos-IriarteUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain.
M M TavíoUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain.
A S RamírezUnidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, C/Trasmontaña s/n, 35413, Arucas, Gran Canaria, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMollicutes detection can be cumbersome due to their slow growth in vitro. For this reason, the use of DNA based on generic molecular tests represents an alternative for rapid, sensitive and specific detection of these microorganism. For this reason, six previously described nucleic acid testing assays were compared to evaluate their ability to detect microorganisms belonging to the class Mollicutes.

methodsA panel of 61 mollicutes, including representatives from the Mycoplasma, Acholeplasma, Mesoplasma, Spiroplasma and Ureaplasma genus, were selected to evaluate the sensitivity and specificity of these assays. A total of 21 non-mollicutes, including closely related non-mollicutes species, were used to evaluate specificity. Limits of detection were calculated to determine the analytical sensitivity of the assays. The two best performing assays were subsequently adapted into real-time PCR format, followed by melting curve analysis.

resultsBoth assays performed satisfactorily, with a 100% specificity described for both assays. The detection limits were found to be between 10

conclusionsThese assays can be very useful for the rapid, specific and sensitive screening cell line contaminants, clinical samples as well as detecting non-culturable, unknown species of mollicutes or mollicutes whose growth is slow or difficult.

Indexed as

Bacteriological TechniquesDNA, BacterialPhytoplasmaReal-Time Polymerase Chain ReactionSensitivity and SpecificityTenericutesDNA, BacterialDiagnosticMollicutesMycoplasmaNatPCRReal-time PCR

Identifiers

PMID28646859
PMCPMC5483278

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.