ArticleVirology2017
Use of Sequence-Independent, Single-Primer-Amplification (SISPA) for rapid detection, identification, and characterization of avian RNA viruses.
Article in Virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
131 citing papers in PubMed, 189 citations in OpenAlex.
- A pegivirus associated with encephalitis in red-legged partridges shows neurotropism across avian species.Nature communications · 2026Article
- Isolation and Identification of Emerging Equine Encephalosis Virus Serotype 6 in Israel, 2023.Pathogens (Basel, Switzerland) · 2026Article
- Characterization of near-complete humanMicrobiology resource announcements · 2026Article
- Genetic diversity and molecular characterization of avian paramyxoviruses from wild birds in South Korea between 2020 and 2024.Frontiers in microbiology · 2026Article
- Evolution and Spread of Regionally Adapted Newcastle Disease Virus Isolates From Live Bird Markets in Nigeria, 2023-2024.Transboundary and emerging diseases · 2026Article
- An amplicon-based tiled PCR scheme for the enrichment of avian metapneumovirus subtype B genomes prior to next generation sequencing.Frontiers in cellular and infection microbiology · 2026Article
- Isolation and whole genome sequencing of an Avian orthoavulavirus 16 from cinereous vulture (Frontiers in veterinary science · 2026Article
- No Evidence of Direct Transmission of Emerging Bluetongue Virus Strains Between Israel and Europe Based on Genomic Analyses (2013-2023).Pathogens (Basel, Switzerland) · 2025Article
- Fecal Sample Surveillance of the Wildlife Virome in Central Italy: Insights from the Foreste Casentinesi National Park.Animals : an open access journal from MDPI · 2025Article
- Phenotypic and genetic changes of Crimean-Congo hemorrhagic fever virus during serial passage in susceptible cell lines.Archives of virology · 2025Article
- Molecular characterization and genotyping of Newcastle disease viruses using whole-genome sequencing from poultry farms in parts of Amhara Regions, and central Ethiopia.Virology journal · 2025Article
- Virome sequencing and analysis of Aedes aegypti and Aedes albopictus from ecologically different sites in the Philippines.Parasites & vectors · 2025Article
- Novel multiplex family-wide PCR and Nanopore sequencing of amplicons (FP-NSA) approach for surveillance of influenza- and coronaviruses in humans and animals.Genome medicine · 2025Article
- Viral Metagenomic Next-Generation Sequencing for One Health Discovery and Surveillance of (Re)Emerging Viruses: A Deep Review.International journal of molecular sciences · 2025Review
- Draft genome sequences of four Newcastle disease virus isolates from commercial poultry farms in Ethiopia.Microbiology resource announcements · 2025Article
- IBDV-SSA, a novel molecular approach for the recovery of infectious bursal disease virus whole genomes from FTA cards.Microbiology spectrum · 2025Article
- Field outbreak investigation and immunoinformatic analysis suggest potential immune evasion by Newcastle disease virus Sub-Genotype XIV.2 in Nigeria.Scientific reports · 2025Article
- Genome sequence of an avian paramyxovirus serotype 14 strain isolated in a chicken in South Korea.Microbiology resource announcements · 2025Article
- Metagenomic Analysis of the Fecal Virome in Wild Mammals Hospitalized in Pisa, Italy.Veterinary sciences · 2025Article
- First documented case of a fatal autochthonous Usutu virus infection in an immunocompromised patient in Hungary: a clinical-virological report and implications from the literature.Virology journal · 2025Article
71 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery of low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combination with MiSeq platform was applied to target negative- and positive-sense single-stranded RNA viral sequences. This method allowed successful sequence assembly of full or near full length avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) viral genome. Moreover, SISPA analysis applied to unknown clinical cases of mixed viral infections produced genome assemblies comprising 98% NDV and 99% of IBV genomes. Complete or near complete virus genome sequence was obtained with titers at or above 10
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.