Evidence map›Paper›PMID 28646449›Full record

ArticleFood and environmental virology2017

Occurrence of Pepper Mild Mottle Virus (PMMoV) in Groundwater from a Karst Aquifer System in the Yucatan Peninsula, Mexico.

Gabriela Rosiles-González, Gerardo Ávila-Torres, Oscar A Moreno-Valenzuela, Gilberto Acosta-González, Rosa María Leal-Bautista, Cinthya D Grimaldo-Hernández, Judith K Brown, Cristóbal Chaidez-Quiroz, Walter Q Betancourt, Charles P Gerba and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Food and environmental virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 38 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Gabriela Rosiles-GonzálezUnidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C., Calle 8, No 39, Mz 29, SM 64, CP 77524, Cancún, Quintana Roo, México.
Gerardo Ávila-TorresUnidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C., Calle 8, No 39, Mz 29, SM 64, CP 77524, Cancún, Quintana Roo, México.
Oscar A Moreno-ValenzuelaUnidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A.C., Calle 43, No 130, Col. Chuburná de Hidalgo, CP 97205, Mérida, Yucatán, México.
Gilberto Acosta-GonzálezUnidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C., Calle 8, No 39, Mz 29, SM 64, CP 77524, Cancún, Quintana Roo, México.
Rosa María Leal-BautistaUnidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C., Calle 8, No 39, Mz 29, SM 64, CP 77524, Cancún, Quintana Roo, México.
Cinthya D Grimaldo-HernándezUnidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C., Calle 8, No 39, Mz 29, SM 64, CP 77524, Cancún, Quintana Roo, México.
Judith K BrownSchool of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA.
Cristóbal Chaidez-QuirozLaboratorio Nacional para la Investigación en Inocuidad Alimentaria, Centro de Investigación en Alimentación y Desarrollo A.C., Carretera a El Dorado Km5.5, Col. Campo El Diez, CP 80129, Culiacán, Sinaloa, México.
Walter Q BetancourtWater and Energy Sustainable Technology (WEST) Center, The University of Arizona, 2959 West Calle Agua Nueva, Tucson, AZ, 85745, USA.
Charles P GerbaWater and Energy Sustainable Technology (WEST) Center, The University of Arizona, 2959 West Calle Agua Nueva, Tucson, AZ, 85745, USA.
Cecilia Hernández-ZepedaUnidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C., Calle 8, No 39, Mz 29, SM 64, CP 77524, Cancún, Quintana Roo, México. cecilia.hernandez@cicy.mx.ORCID 0000-0003-4145-4116
University of Arizona · USCentro de Estudios Científicos · CLCentro de Investigación en Alimentación y Desarrollo · MXSecretaría de Investigación, Innovación y Educación Superior · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Yucatan Peninsula of Mexico hosts a karst aquifer system that is the only source of freshwater for the area; however, it is vulnerable to human-mediated contamination. Pepper mild mottle virus (PMMoV) is one of the most abundant RNA viruses associated with human feces, making it a viable indicator for tracking fecal pollution in aquatic environments, including groundwater. In this study, groundwater samples collected from a karst aquifer from fresh and brackish water locations were analyzed for fecal indicator bacteria, somatic and male F+ specific coliphages, and PMMoV during the rainy and dry seasons. Total coliform bacteria were detected at all sites, whereas Escherichia coli were found at relatively low levels <40 MPN/100 ml. The highest average concentrations of somatic and male F+ specific coliphages were 920 and 330 plaque forming units per 100 ml, respectively, detected in freshwater during the rainy season. PMMoV RNA was detected in 85% of the samples with gene sequences sharing 99-100% of nucleotide identity with PMMoV sequences available in GenBank. Quantification of PMMoV genome copies (GC) by quantitative real-time PCR indicated concentrations ranging from 1.7 × 10

Indexed as

BacteriaFecesGroundwaterMexicoReal-Time Polymerase Chain ReactionSeasonsTobamovirusFecal indicatorGroundwaterKarstPepper mild mottle virusYucatan Peninsula

Identifiers

PMID28646449
OpenAlexW2712753142

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.