ArticleMolecular and cellular biochemistry2018
Lead enhancement of 3T3-L1 fibroblasts differentiation to adipocytes involves ERK, C/EBPβ and PPARγ activation.
Article in Molecular and cellular biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Research progress on the regulatory mechanism of cell senescence in arsenic toxicity: a systematic review.Toxicology research · 2024Review
- SSTR2 Mediates the Inhibitory Effect of SST/CST on Lipolysis in Chicken Adipose Tissue.Animals : an open access journal from MDPI · 2024Article
- Assessing the potential molecular mechanism of arsenite-induced skin cell senescence.Toxicology research · 2023Article
- Positive Regulation of Acetate in Adipocyte Differentiation and Lipid Deposition in Obese Mice.Nutrients · 2023Article
- Adipogenic and endocrine disrupting mixture effects of organic and inorganic pollutant mixtures.The Science of the total environment · 2023Article
- Review
- Differential fat accumulation in early adulthood according to adolescent-BMI and heavy metal exposure.New directions for child and adolescent development · 2022Article
- Assessing the Potential Value and Mechanism ofOxidative medicine and cellular longevity · 2022Article
- Machine-learning models predicting osteoarthritis associated with the lead blood level.Environmental science and pollution research international · 2021Article
- Characterization of adipogenic, PPARγ, and TRβ activities in house dust extracts and their associations with organic contaminants.The Science of the total environment · 2021Article
- Adipotropic effects of heavy metals and their potential role in obesity.Faculty reviews · 2021Review
- Effect of lead on proliferation, oxidative stress and genotoxic damage of 3T3-L1 fibroblasts.Toxicology research · 2020Article
- Adipogenesis Regulation and Endocrine Disruptors: Emerging Insights in Obesity.BioMed research international · 2020Review
- The Antiobesity Effects of Buginawa in 3T3-L1 Preadipocytes and in a Mouse Model of High-Fat Diet-Induced Obesity.BioMed research international · 2019Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Lead (Pb) is an environmental and industrial contaminant that still represents a public health problem. Elevated Pb exposure has been inversely correlated with femoral bone density and associated with osteoporosis. In the last years, it has been shown that inhibition of osteogenesis from mesenchymal stem cells activates adipogenesis and vice versa. In this paper, we investigated the effect of Pb on the differentiation of 3T3-L1 fibroblasts to adipocytes which is the cell model most used to study adipogenesis. After induction of differentiation, 2 days post-confluent cells re-enter the cell cycle and undergo mitotic clonal expansion (MCE) followed by expression of genes that produce the adipocyte phenotype. The presence of concentrations of Pb up to 10 μM during differentiation of 3T3-L1 fibroblasts did not interfere with MCE but enhanced the accumulation of cytosolic lipids that occur during adipogenesis, as well as, the induction of PPARγ, the master gene in adipogenesis. It is known that PPARγ upregulation is subsequent to induction of C/EBPβ and ERK activation, which are early events in adipogenesis. We found that both events were enhanced by Pb treatment. Our results support a stimulatory effect of Pb on adipogenesis which involves ERK activation and C/EBPβ upregulation prior to PPARγ and adipogenesis activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.