ArticleScientific reports2017
Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A hormone-dependent tRNA half promotes cell cycle progression via destabilization of p21 mRNA.PLoS biology · 2025Article
- Landscape of small nucleic acid therapeutics: moving from the bench to the clinic as next-generation medicines.Signal transduction and targeted therapy · 2025Review
- Review
- The NHE3 Inhibitor Tenapanor Prevents Intestinal Obstructions in CFTR-Deleted Mice.International journal of molecular sciences · 2022Article
- Article
- Accurate quantification of 3'-terminal 2'-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR.Frontiers of medicine · 2022Article
- RNase κ promotes robust piRNA production by generating 2',3'-cyclic phosphate-containing precursors.Nature communications · 2021Article
- Article
- Oxidative stress enhances the expression of 2',3'-cyclic phosphate-containing RNAs.RNA biology · 2020Article
- tRNA Fragments Populations Analysis in Mutants Affecting tRNAs Processing and tRNA Methylation.Frontiers in genetics · 2020Article
- Short RNA regulators: the past, the present, the future, and implications for precision medicine and health disparities.Current opinion in biotechnology · 2019Review
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Authors and funding
8 authors.
Funding
Abstract
Piwi proteins and their bound Piwi-interacting RNAs (piRNAs) are predominantly expressed in the germline and play crucial roles in germline development by silencing transposons and other targets. Bombyx mori BmN4 cells are culturable germ cells that equip the piRNA pathway. Because of the scarcity of piRNA-expressing culturable cells, BmN4 cells are being utilized for the analyses of piRNA biogenesis. We here report that the piRNA biogenesis in BmN4 cells is regulated by cell density. As cell density increased, the abundance of Piwi proteins and piRNA biogenesis factors was commonly upregulated, resulting in an increased number of perinuclear nuage-like granules where Piwi proteins localize. Along with these phenomena, the abundance of mature piRNAs also globally increased, whereas levels of long piRNA precursor and transposons decreased, suggesting that increasing cell density promotes piRNA biogenesis pathway and that the resultant accumulation of mature piRNAs is functionally significant for transposon silencing. Our study reveals a previously uncharacterized link between cell density and piRNA biogenesis, designates cell density as a critical variable in piRNA studies using BmN4 cell system, and suggests the alteration of cell density as a useful tool to monitor piRNA biogenesis and function.
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Registered trials
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