Evidence map›Paper›PMID 28645377›Full record

ArticleMethods in enzymology2017

Electrical Probes of DNA-Binding Proteins.

Jacqueline K Barton, Phillip L Bartels, Yingxin Deng, Elizabeth O'Brien

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Sensing DNA through DNA Charge Transport.ACS chemical biology · 2018
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jacqueline K BartonCalifornia Institute of Technology, Pasadena, CA, United States. Electronic address: jkbarton@caltech.edu.
Phillip L BartelsCalifornia Institute of Technology, Pasadena, CA, United States.
Yingxin DengCalifornia Institute of Technology, Pasadena, CA, United States.
Elizabeth O'BrienCalifornia Institute of Technology, Pasadena, CA, United States.

Funding

ELECTROCHEMICAL DNA-BASED SENSORSR01GM061077 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI BARTON, JACQUELINE K · 2000 to 2017
$5.2M
NIGMS NIH HHS R01 GM061077
6 · The paper itself

Abstract

A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins. For redox-active proteins, the electrochemistry permits the determination of redox potentials in the DNA-bound form, where comparisons to DNA-free potentials can be made using graphite electrodes without DNA modification. Importantly, electrochemistry on the DNA-modified electrodes facilitates reaction under aqueous, physiological conditions with a sensitive electrical measurement of binding and activity.

Indexed as

Molecular ProbesClick ChemistryDNA-Binding ProteinsElectrodesOxidation-ReductionDNA-Binding ProteinsMolecular ProbesBase excision repairBase flippingDNA alkylation repairDNA bindingDNA glycosylaseDNA modificationKinetic simulationNucleotide flippingStopped flowTransient kinetics

Identifiers

PMID28645377
PMCPMC6314295

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.