ArticleMethods in enzymology2017
Ensemble and Single-Molecule Analysis of Non-Homologous End Joining in Frog Egg Extracts.
Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Core NHEJ Factors Maintain Synapsis Across Diverse DNA End Structures.bioRxiv : the preprint server for biology · 2026Article
- DNA polymerase λ autoinhibition is relieved via Ku interaction during non-homologous end joining.Nucleic acids research · 2026Article
- Conformational regulation of two essential activators of bacterial cell elongation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The hit-and-run of cell wall synthesis: LpoB transiently binds and activates PBP1b through a conserved allosteric switch.Nature communications · 2025Article
- MreC-MreD structure reveals a multifaceted interface that controls MreC conformation.bioRxiv : the preprint server for biology · 2024Article
- Identification of the main barriers to Ku accumulation in chromatin.Cell reports · 2024Article
- Structural role for DNA Ligase IV in promoting the fidelity of non-homologous end joining.Nature communications · 2024Article
- Identification of the main barriers to Ku accumulation in chromatin.bioRxiv : the preprint server for biology · 2024Article
- Review
- Allosteric activation of cell wall synthesis during bacterial growth.Nature communications · 2023Article
- H2A.Z deposition by SWR1C involves multiple ATP-dependent steps.Nature communications · 2022Article
- V(D)J Recombination: Recent Insights in Formation of the Recombinase Complex and Recruitment of DNA Repair Machinery.Frontiers in cell and developmental biology · 2022Review
- Mechanistic Insights From Single-Molecule Studies of Repair of Double Strand Breaks.Frontiers in cell and developmental biology · 2021Review
- Article
- A Mechanism to Minimize Errors during Non-homologous End Joining.Molecular cell · 2020Article
- Expanding single-molecule fluorescence spectroscopy to capture complexity in biology.Current opinion in structural biology · 2019Review
- The Gene-Silencing Protein MORC-1 Topologically Entraps DNA and Forms Multimeric Assemblies to Cause DNA Compaction.Molecular cell · 2019Article
- A Drosophila cell-free system that senses DNA breaks and triggers phosphorylation signalling.Nucleic acids research · 2019Article
- A single XLF dimer bridges DNA ends during nonhomologous end joining.Nature structural & molecular biology · 2018Article
- The flexible and iterative steps within the NHEJ pathway.Progress in biophysics and molecular biologyArticle
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Authors and funding
3 authors.
Funding
Abstract
Non-homologous end joining (NHEJ) repairs the majority of DNA double-strand breaks in human cells, yet the detailed order of events in this process has remained obscure. Here, we describe how to employ Xenopus laevis egg extract for the study of NHEJ. The egg extract is easy to prepare in large quantities, and it performs efficient end joining that requires the core end joining proteins Ku, DNA-PKcs, XLF, XRCC4, and DNA ligase IV. These factors, along with the rest of the soluble proteome, are present at endogenous concentrations, allowing mechanistic analysis in a system that begins to approximate the complexity of cellular end joining. We describe an ensemble assay that monitors covalent joining of DNA ends and fluorescence assays that detect joining of single pairs of DNA ends. The latter assay discerns at least two discrete intermediates in the bridging of DNA ends.
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