Evidence map›Paper›PMID 28643224›Full record

ArticleIn vitro cellular & developmental biology. Animal2017

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk.

Maria Hillreiner, Nadine I Müller, Heiner M Koch, Christiane Schmautz, Bernhard Küster, Michael W Pfaffl, Heike Kliem

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Maria HillreinerChair of Animal Physiology and Immunology, Technische Universität München, Freising, Germany.
Nadine I MüllerChair of Animal Physiology and Immunology, Technische Universität München, Freising, Germany.
Heiner M KochChair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.
Christiane SchmautzChair of Animal Physiology and Immunology, Technische Universität München, Freising, Germany.
Bernhard KüsterChair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany.
Michael W PfafflChair of Animal Physiology and Immunology, Technische Universität München, Freising, Germany.
Heike KliemChair of Animal Physiology and Immunology, Technische Universität München, Freising, Germany. kliem@wzw.tum.de.
Technical University of Munich · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

For the investigation of molecular processes underlying diseases of the bovine mammary gland, primary bovine mammary epithelial cells (pbMEC) are used. They are known to contribute to the innate immune system of the bovine mammary gland. The functionality of pbMEC depends on the maintenance of in vivo characteristics. So far, the optimization of pbMEC culture conditions was intended in a variety of experiments. For this purpose, most of the studies used stable cell lines or primary cells obtained from udder biopsies of slaughtered animals. By contrast, within our study, pbMEC of healthy and first lactating Brown Swiss cows were non-invasively isolated from fresh milk. The non-invasively isolated pbMEC were cultivated on the extracellular matrix-like scaffold Matrigel®

Indexed as

AnimalsCattleCell Culture TechniquesEpithelial CellsFemaleLactationMammary Glands, AnimalMilkMilk ProteinsPrimary Cell CultureMilk Proteins3D cell cultureExtracellular matrixLactogenesisPrimary bovine mammary epithelial cellsProteomicsRT-qPCR

Identifiers

PMID28643224
OpenAlexW2691426509

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.