Evidence map›Paper›PMID 28643089›Full record

ArticleJournal of assisted reproduction and genetics2017

Time-lapse observation and transcriptome analysis of a case with repeated multiple pronuclei after IVF/ICSI.

J Dai, L Z Leng, C F Lu, F Gong, S P Zhang, W Zheng, G X Lu, G Lin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Advances in the genetic etiology of female infertility.Journal of assisted reproduction and genetics · 2024
    Review
  4. Review
  5. The Application of Single-Cell RNA Sequencing in Mammalian Meiosis Studies.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

J DaiReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.
L Z LengInstitute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Changsha, China.
C F LuReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.
F GongReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.
S P ZhangReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.
W ZhengReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.
G X LuReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China.
G LinReproductive and Genetic Hospital of CITIC-XIANGYA, Changsha, China. linggf@hotmail.com.
Central South University · CNReproductive & Genetic Hospital CITIC-Xiangya · CNNational Engineering Research Center of Human Stem Cells · CN

Funding

Program for New Century Excellent Talents in University No. 907010003the Fundamental Research Funds for the Central Universities of Central South University No. 2015zzts101the National Natural Science Foundation of China No. 81222007the National Natural Science Foundation of China No. 81471510
6 · The paper itself

Abstract

purposeThe purpose of this study was to investigate the cause of repeated multipronucleus (MPN) formation in zygotes in a patient after both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).

methodThis is a case study. A patient had unexplained primary infertility with recurring total MPN zygotes after IVF and ICSI cycles. Time-lapse monitoring of pronucleus formation was carried out. Embryos developed from MPN zygotes were analyzed by fluorescence in situ hybridization (FISH). Single-cell RNA-seq analysis was used to identify gene expression profiles of the patient's oocyte and zygote, and these were compared to the data from oocytes and zygotes from donors with normal fertilization (patient, n = 1; donors, n = 4). Oocyte-specific genes with differential expression were selected by the Amazonia! DATABASE:

resultsFrom time-lapse analysis, we observed the formation of multiple micronuclei near the site of the second polar body extrusion. These micronuclei migrated, expanded, and juxtaposed with the male pronucleus leading to a multipronucleus. None of these MPN zygotes could develop to the blastocyst stage, and FISH analysis revealed a chaotic chromosomal complement in the arrested embryos. RNA-seq analysis showed 113 differentially expressed genes (DEGs) between the patient and the donor oocytes and zygotes. Moreover, 25 of the 113 DEGs were unique or highly expressed in oocytes and early embryos. From 25 DEGs, three genes, DYNC2LI1, NEK2, and CCNH, which are involved in meiosis and the chromosome separation process, were further validated by real-time PCR.

conclusionWe identified several candidate genes affecting pronucleus formation as a new cause of infertility.

Indexed as

Fertilization in VitroGene Expression ProfilingCell NucleusFemaleHumansInfertilityIn Situ Hybridization, FluorescenceMaleMeiosisOocytesSperm Injections, IntracytoplasmicTime-Lapse ImagingTranscriptomeZygoteMultipronuclearOocyteSingle-cell transcriptomeTime-lapse

Identifiers

PMID28643089
PMCPMC5581788
OpenAlexW2662476987

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.