Evidence map›Paper›PMID 28642487›Full record

ArticleScientific reports2017

Hypoxia is a Key Driver of Alternative Splicing in Human Breast Cancer Cells.

Jian Han, Jia Li, Jolene Caifeng Ho, Grace Sushin Chia, Hiroyuki Kato, Sudhakar Jha, Henry Yang, Lorenz Poellinger, Kian Leong Lee

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed, 1 pooled it
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.

  1. Alternative splicing and cancer: a systematic review.Signal transduction and targeted therapy · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Jian HanCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Jia LiCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Jolene Caifeng HoCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.ORCID 0000-0001-5908-0007
Grace Sushin ChiaCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Hiroyuki KatoCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Sudhakar JhaCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Henry YangCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Lorenz PoellingerCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore.
Kian Leong LeeCancer Science Institute of Singapore, National University of Singapore, 117599, Singapore, Singapore. csilkl@nus.edu.sg.ORCID 0000-0002-0443-2746
National University of Singapore · SG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adaptation to hypoxia, a hallmark feature of many tumors, is an important driver of cancer cell survival, proliferation and the development of resistance to chemotherapy. Hypoxia-induced stabilization of hypoxia-inducible factors (HIFs) leads to transcriptional activation of a network of hypoxia target genes involved in angiogenesis, cell growth, glycolysis, DNA damage repair and apoptosis. Although the transcriptional targets of hypoxia have been characterized, the alternative splicing of transcripts that occurs during hypoxia and the roles they play in oncogenesis are much less understood. To identify and quantify hypoxia-induced alternative splicing events in human cancer cells, we performed whole transcriptome RNA-Seq in breast cancer cells that are known to provide robust transcriptional response to hypoxia. We found 2005 and 1684 alternative splicing events including intron retention, exon skipping and alternative first exon usage that were regulated by acute and chronic hypoxia where intron retention was the most dominant type of hypoxia-induced alternative splicing. Many of these genes are involved in cellular metabolism, transcriptional regulation, actin cytoskeleton organisation, cancer cell proliferation, migration and invasion, suggesting they may modulate or be involved in additional features of tumorigenic development that extend beyond the known functions of canonical full-length transcripts.

Indexed as

Alternative SplicingGene Expression Regulation, NeoplasticBreast NeoplasmsCell Line, TumorCell MovementCell SurvivalExonsFemaleGene Expression ProfilingGTPase-Activating ProteinsHumansHypoxiaIntronsMembrane ProteinsNeoplasm ProteinsNF-E2-Related Factor 1AHNAK protein, humanARHGAP4 protein, humanGTPase-Activating ProteinsMembrane ProteinsNeoplasm ProteinsNFE2L1 protein, humanNF-E2-Related Factor 1TNFSF13 protein, humanTranscription FactorsTumor Necrosis Factor Ligand Superfamily Member 13VGLL4 protein, human

Identifiers

PMID28642487
PMCPMC5481333
OpenAlexW2626865717

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.