ArticleScience (New York, N.Y.)2017
Metalloprotein entatic control of ligand-metal bonds quantified by ultrafast x-ray spectroscopy.
Article in Science (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 139 citations in OpenAlex.
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- Femtosecond Soft X-ray Absorption Spectroscopy Identifies Metal-Centered SJournal of the American Chemical Society · 2026Article
- Multiplet lines in seeded stimulated MnPhysical review applied · 2026Article
- Proton-linked effects on the axial coordination in ferric and ferrous horse heart cytochrome c.Protein science : a publication of the Protein Society · 2025Article
- Disabling the Entatic Control of Methionine Ligation through Additive Destabilization of Ferric CytochromeInorganic chemistry · 2025Article
- A high-energy Laue X-ray emission spectrometer at the FXE instrument at the European XFEL.Journal of synchrotron radiation · 2025Article
- X-ray Absorption Spectroscopy of Dilute Metalloenzymes at X-ray Free-Electron Lasers in a Shot-by-Shot Mode.The journal of physical chemistry letters · 2025Article
- Stimulated X-ray emission spectroscopy.Photosynthesis research · 2024Review
- XFEL Beamline Optical Instrumentation for Ultrafast Science.The journal of physical chemistry. B · 2024Review
- The Liquid Jet Endstation for Hard X-ray Scattering and Spectroscopy at the Linac Coherent Light Source.Molecules (Basel, Switzerland) · 2024Article
- Experimental capabilities for liquid jet samples at sub-MHz rates at the FXE Instrument at European XFEL.Journal of synchrotron radiation · 2023Article
- In situ X-ray spectroscopies beyond conventional X-ray absorption spectroscopy on deciphering dynamic configuration of electrocatalysts.Nature communications · 2023Review
- Genetically Encoded Fluorescent Probe for Detection of Heme-Induced Conformational Changes in Cytochrome c.Biosensors · 2023Article
- Solution phase high repetition rate laser pump x-ray probe picosecond hard x-ray spectroscopy at the Stanford Synchrotron Radiation Lightsource.Structural dynamics (Melville, N.Y.) · 2023Article
- The role of basicity in selective C-H bond activation by transition metal-oxidos.Dalton transactions (Cambridge, England : 2003) · 2023Review
- Multicomponent X-ray Shielding Using Sulfated Cerium Oxide and Bismuth Halide Composites.Molecules (Basel, Switzerland) · 2023Article
- Room temperature X-ray absorption spectroscopy of metalloenzymes with drop-on-demand sample delivery at XFELs.Pure and applied chemistry. Chimie pure et appliquee · 2023Article
- Ferricyanide photo-aquation pathway revealed by combined femtosecond Kβ main line and valence-to-core x-ray emission spectroscopy.Nature communications · 2023Article
- Sensitivity of Kβ mainline X-ray emission to structural dynamics in iron photosensitizer.Physical chemistry chemical physics : PCCP · 2023Article
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Authors and funding
17 authors at 2 institutions in 1 country.
Funding
Abstract
The multifunctional protein cytochrome c (cyt c) plays key roles in electron transport and apoptosis, switching function by modulating bonding between a heme iron and the sulfur in a methionine residue. This Fe-S(Met) bond is too weak to persist in the absence of protein constraints. We ruptured the bond in ferrous cyt c using an optical laser pulse and monitored the bond reformation within the protein active site using ultrafast x-ray pulses from an x-ray free-electron laser, determining that the Fe-S(Met) bond enthalpy is ~4 kcal/mol stronger than in the absence of protein constraints. The 4 kcal/mol is comparable with calculations of stabilization effects in other systems, demonstrating how biological systems use an entatic state for modest yet accessible energetics to modulate chemical function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.