Evidence map›Paper›PMID 28640893›Full record

ArticlePloS one2017

Oroxylin A promotes retinal ganglion cell survival in a rat optic nerve crush model.

Shu-Fang Lin, Jia-Ying Chien, Kishan Kapupara, Chi-Ying F Huang, Shun-Ping Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Shu-Fang LinInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Jia-Ying ChienDepartment of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.
Kishan KapuparaDepartment of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.
Chi-Ying F HuangInstitute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Shun-Ping HuangDepartment of Molecular Biology and Human Genetics, Tzu Chi University, Hualien, Taiwan.
National Yang Ming Chiao Tung University · TWTzu Chi University · TWNational Tsing Hua University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate the effect of oroxylin A on the survival of retinal ganglion cells (RGC) and the activation of microglial cells in a rat optic nerve (ON) crush model.

methodsOroxylin A (15mg/Kg in 0.2ml phosphate-buffered saline) or phosphate-buffered saline (PBS control) was immediately administered after ON crush once by subcutaneous injection. Rats were euthanized at 2 weeks after the crush injury. The density of RGC was counted by retrograde labeling with FluoroGold and immunostaining of retina flat mounts for Brn3a. Electrophysiological visual function was assessed by flash visual evoked potentials (FVEP). TUNEL assay, immunoblotting analysis of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the retinas, and immunohistochemistry of GFAP in the retinas and ED1 in the ON were evaluated.

resultsTwo weeks after the insult, the oroxylin A-treated group had significantly higher FG labeled cells and Brn3a+ cells suggesting preserved RGC density in the central and mid-peripheral retinas compared with those of the PBS-treated group. FVEP measurements showed a significantly better preserved latency of the P1 wave in the ON-crushed, oroxylin A-treated rats than the ON-crushed, PBS treated rats. TUNEL assays showed fewer TUNEL positive cells in the ON-crushed, oroxylin A-treated rats. The number of ED1 positive cells was reduced at the lesion site of the optic nerve in the ON-crushed, oroxylin A-treated group. Increased GFAP expression in the retina was reduced greatly in ON-crushed, oroxylin A-treated group. Furthermore, administration of oroxylin A significantly attenuated ON crush insult-induced iNOS and COX-2 expression in the retinas.

conclusionsThese results demonstrated that oroxylin A hasss neuroprotective effects on RGC survival with preserved visual function and a decrease in microglial infiltration in the ONs after ON crush injury.

Indexed as

AnimalsCell SurvivalCyclooxygenase 2CytokinesDisease Models, AnimalEvoked Potentials, VisualFlavonoidsGene Expression Regulation, EnzymologicMaleMicrogliaNitric Oxide Synthase Type IIOptic Nerve InjuriesRatsRats, WistarRetinal Ganglion CellsUp-Regulation5,7-dihydroxy-6-methoxy-2-phenylchromen-4-oneCyclooxygenase 2CytokinesFlavonoidsNitric Oxide Synthase Type II

Identifiers

PMID28640893
PMCPMC5480866
OpenAlexW2656686091

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.