ArticlePloS one2017
Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- NcSWP8, a New Spore Wall Protein, Interacts with Polar Tube Proteins in the Parasitic MicrosporidiaMicroorganisms · 2025Article
- In Vitro Cultivation forMicroorganisms · 2024Article
- Microsporidian Nosema bombycis hijacks host vitellogenin and restructures ovariole cells for transovarial transmission.PLoS pathogens · 2023Article
- Ssn6 Interacts with Polar Tube Protein 2 and Transcriptional Repressor for RNA Polymerase II: Insight into Its Involvement in the Biological Process of MicrosporidiumJournal of fungi (Basel, Switzerland) · 2023Article
- A Putative TRAPα Protein of MicrosporidiaJournal of fungi (Basel, Switzerland) · 2023Article
- Characterizing the Proliferation Patterns of Representative Microsporidian Species Enlightens Future Studies of Infection Mechanisms.Pathogens (Basel, Switzerland) · 2022Article
- Heterologous Expressed NbSWP12 from MicrosporidiaJournal of fungi (Basel, Switzerland) · 2022Article
- Stable transformation of fluorescent proteins into Nosema bombycis by electroporation.Parasites & vectors · 2022Article
- Identification and subcellular localization analysis of membrane protein Ycf 1 in the microsporidianPeerJ · 2022Article
- Engineered resistance to Nosema bombycis by in vitro expression of a single-chain antibody in Sf9-III cells.PloS one · 2018Article
- Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while β-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.