Evidence map›Paper›PMID 28638047›Full record

ArticleScientific reports2017

Semi-rational engineering of a thermostable aldo-keto reductase from Thermotoga maritima for synthesis of enantiopure ethyl-2-hydroxy-4-phenylbutyrate (EHPB).

Zhiguo Wang, Shuo Zhou, Shuangling Zhang, Sa Zhang, Fangmeng Zhu, Xiaolu Jin, Zhenming Chen, Xiaoling Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zhiguo WangHangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Shuo ZhouHangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Shuangling ZhangHangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Sa ZhangHangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Fangmeng ZhuApeloa Pharmaceutical Co., Ltd., Dongyang, Zhejiang, 322118, China.
Xiaolu JinYosemade Pharmaceutical Co., Ltd., Jinhua, Zhejiang, 321025, China.
Zhenming ChenHangzhou Normal University, Hangzhou, Zhejiang, 311121, China. zchenhznu@126.com.
Xiaoling XuHangzhou Normal University, Hangzhou, Zhejiang, 311121, China. xuxl@hznu.edu.cn.
Hangzhou Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel aldo-keto reductase Tm1743 characterized from Thermotoga maritima was explored as an effective biocatalyst in chiral alcohol production. Natural Tm1743 catalyzes asymmetric reduction of ethyl 2-oxo-4-phenylbutyrate (EOPB) at high efficiency, but the production of, ethyl (S)-2-hydroxy-4-phenylbutyrate ((S)-EHPB), which is less desirable, is preferred with an enantiomeric excess (ee) value of 76.5%. Thus, altering the enantioselectivity of Tm1743 to obtain the more valuable product (R)-EHPB for angiotensin drug synthesis is highly desired. In this work, we determined the crystal structure of Tm1743 in complex with its cofactor NADP

Indexed as

Protein EngineeringAldo-Keto ReductasesCrystallography, X-RayEnzymesEnzyme StabilityHot TemperatureMutagenesisPhenylbutyratesSubstrate SpecificityThermotoga maritimaAldo-Keto ReductasesEnzymesethyl 2-oxo-4-phenylbutyratePhenylbutyrates

Identifiers

PMID28638047
PMCPMC5479831
OpenAlexW2625685343

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.