Evidence map›Paper›PMID 28637896›Full record

ArticleJournal of leukocyte biology2017

Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate.

Phillip J Stewart-Hutchinson, Taylor P Szasz, Emily R Jaeger, Michael D Onken, John A Cooper, Sharon Celeste Morley

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of leukocyte biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Phillip J Stewart-HutchinsonDivision of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
Taylor P SzaszDivision of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
Emily R JaegerDivision of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
Michael D OnkenDepartments of Biochemistry and Molecular Biophysics and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA; and.
John A CooperDepartments of Biochemistry and Molecular Biophysics and Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA; and.
Sharon Celeste MorleyDivision of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA; morley_c@kids.wustl.edu.
Washington University in St. Louis · US

Funding

ACTIN ASSEMBLY AND CELL MOTILITY: MECHANISMS AND REGULATIONR35GM118171 · NIGMS · WASHINGTON UNIVERSITY · PI COOPER, JOHN A · 2016 to 2020
$2.8M
CONTROL OF ADAPTIVE IMMUNITY BY ACTIN-REGULATORY PROTEINSR01AI104732 · NIAID · WASHINGTON UNIVERSITY · PI MORLEY, SHARON CELESTE · 2014 to 2018
$1.9M
NIAID NIH HHS R01 AI104732NIGMS NIH HHS R35 GM118171
6 · The paper itself

Abstract

Migration of B cells supports their development and recruitment into functional niches. Therefore, defining factors that control B cell migration will lead to a better understanding of adaptive immunity. In vitro cell migration assays with B cells have been limited by poor adhesion of cells to glass coated with adhesion molecules. We have developed a technique using monolayers of endothelial cells as the substrate for B cell migration and used this technique to establish a robust in vitro assay for B cell migration. We use TNF-α to up-regulate surface expression of the adhesion molecule VCAM-1 on endothelial cells. The ligand VLA-4 is expressed on B cells, allowing them to interact with the endothelial monolayer and migrate on its surface. We tested our new method by examining the role of L-plastin (LPL), an F-actin-bundling protein, in B cell migration. LPL-deficient (LPL

Indexed as

AnimalsB-LymphocytesCell MovementCoculture TechniquesCytoskeletal ProteinsEndothelial CellsIntegrin alpha4beta1MiceMice, KnockoutMicrofilament ProteinsPhosphoproteinsTumor Necrosis Factor-alphaCytoskeletal ProteinsIntegrin alpha4beta1Lcp1 protein, mouseMicrofilament ProteinsPhosphoproteinsTumor Necrosis Factor-alphaactin cytoskeletonB cell migrationB lymphocytesL-plastin

Identifiers

PMID28637896
PMCPMC5557643
OpenAlexW2698853811

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.