ArticleMicrobial cell factories2017
Improved γ-linolenic acid production in Mucor circinelloides by homologous overexpressing of delta-12 and delta-6 desaturases.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Microbial cell factories for gamma-linolenic acid biosynthesis: metabolic engineering, downstream processing, and future perspectives.Bioresources and bioprocessing · 2026Review
- Nicotinamide and Sodium Butyrate Double the Pool of Treatment-Exclusive Metabolites in the Endophytic Fungus Curvularia sp. G6-32.Chemistry & biodiversity · 2026Article
- Fatty acid synthase in high and low lipid-producing strains of Mucor circinelloides: identification, phylogenetic analysis, and expression profiling during growth and lipid accumulation.Biotechnology letters · 2025Article
- Proteomic insights into the physiology and metabolism of oleaginous yeasts and filamentous fungi.Frontiers in microbiology · 2025Review
- Recent advances and future directions on GLA-producing organisms.Frontiers in bioengineering and biotechnology · 2025Review
- Lipid production from corn straw by cellobiohydrolase and delta-6 desaturase engineered Mucor circinelloides strains under solid state fermentation.Scientific reports · 2024Article
- Symmetric and asymmetric DNA N6-adenine methylation regulates different biological responses in Mucorales.Nature communications · 2024Article
- Dissecting Holistic Metabolic Acclimatization ofBiology · 2024Article
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.Frontiers in nutrition · 2022Review
- Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production inFrontiers in nutrition · 2022Article
- Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi.Frontiers in nutrition · 2022Review
- Overexpression of the Mitochondrial Malic Enzyme Genes (Frontiers in microbiology · 2022Article
- Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid inFrontiers in microbiology · 2022Article
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2.Scientific reports · 2021Article
- Lipid Accumulation by Xylose Metabolism Engineered Mucor circinelloides Strains on Corn Straw Hydrolysate.Applied biochemistry and biotechnology · 2021Article
- Role of Snf-β in lipid accumulation in the high lipid-producing fungus Mucor circinelloides WJ11.Microbial cell factories · 2021Article
- Heterologous Expression of Two Malate Transporters From an Oleaginous FungusFrontiers in microbiology · 2021Article
- Tricarboxylate Citrate Transporter of an Oleaginous FungusFrontiers in nutrition · 2021Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundγ-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA. Thus, it is necessary to explore the main contributing factor during the process of GLA biosynthesis in M. circinelloides.
resultsTo enhance GLA production in M. circinelloides, homologous overexpression of delta-12 and two delta-6 desaturases (named delta-6-1 and delta-6-2, respectively) were analyzed. When delta-6 desaturase were overexpressed in M. circinelloides, up to 43% GLA was produced in the total fatty acids, and the yield of GLA reached 180 mg/l, which were, respectively, 38 and 33% higher than the control strain.
conclusionThese findings revealed that delta-6 desaturase (especially for delta-6-1 desaturase) plays an important role in GLA synthesis by M. circinelloides. The strain overexpressing delta-6-1 desaturase may have potential application in microbial GLA production.
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