ArticleNucleic acids research2017
Identification of Arabidopsis thaliana upstream open reading frames encoding peptide sequences that cause ribosomal arrest.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Translational Regulation of Plant Stress Responses: Mechanisms, Pathways, and Applications in Bioengineering.Annual review of phytopathology · 2025Review
- What, where, and how: Regulation of translation and the translational landscape in plants.The Plant cell · 2024Review
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Plant translational reprogramming for stress resilience.Frontiers in plant science · 2023Review
- Plants utilise ancient conserved peptide upstream open reading frames in stress-responsive translational regulation.Plant, cell & environment · 2022Article
- Microproteins in skeletal muscle: hidden keys in muscle physiology.Journal of cachexia, sarcopenia and muscle · 2022Review
- Upstream Open Reading Frame Mediated Translation of WNK8 Is Required for ABA Response inInternational journal of molecular sciences · 2021Article
- The role of RST1 and RIPR proteins in plant RNA quality control systems.Plant molecular biology · 2021Article
- Application of Upstream Open Reading Frames (uORFs) Editing for the Development of Stress-Tolerant Crops.International journal of molecular sciences · 2021Review
- Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames.Nucleic acids research · 2021Article
- Determinants of genome-wide distribution and evolution of uORFs in eukaryotes.Nature communications · 2021Article
- Translational gene regulation in plants: A green new deal.Wiley interdisciplinary reviews. RNA · 2020Review
- uORFs: ImportantInternational journal of molecular sciences · 2020Review
- uORF Shuffling Fine-Tunes Gene Expression at a Deep Level of the Process.Plants (Basel, Switzerland) · 2020Review
- Comprehensive genome-wide identification of angiosperm upstream ORFs with peptide sequences conserved in various taxonomic ranges using a novel pipeline, ESUCA.BMC genomics · 2020Article
- Reverse genetics-based biochemical studies of the ribosomal exit tunnel constriction region in eukaryotic ribosome stalling: spatial allocation of the regulatory nascent peptide at the constriction.Nucleic acids research · 2020Article
- Metabolite Control of Translation by Conserved Peptide uORFs: The Ribosome as a Metabolite Multisensor.Plant physiology · 2020Article
- Multi-strategic RNA-seq analysis reveals a high-resolution transcriptional landscape in cotton.Nature communications · 2019Article
- Computational and Experimental Tools to Monitor the Changes in Translation Efficiency of Plant mRNA on a Genome-Wide Scale: Advantages, Limitations, and Solutions.International journal of molecular sciences · 2018Review
- Boron-Dependent Translational Suppression of the Borate ExporterPlant physiology · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specific sequences of certain nascent peptides cause programmed ribosomal arrest during mRNA translation to control gene expression. In eukaryotes, most known regulatory arrest peptides are encoded by upstream open reading frames (uORFs) present in the 5'-untranslated region of mRNAs. However, to date, a limited number of eukaryotic uORFs encoding arrest peptides have been reported. Here, we searched for arrest peptide-encoding uORFs among Arabidopsis thaliana uORFs with evolutionarily conserved peptide sequences. Analysis of in vitro translation products of 22 conserved uORFs identified three novel uORFs causing ribosomal arrest in a peptide sequence-dependent manner. Stop codon-scanning mutagenesis, in which the effect of changing the uORF stop codon position on the ribosomal arrest was examined, and toeprint analysis revealed that two of the three uORFs cause ribosomal arrest during translation elongation, whereas the other one causes ribosomal arrest during translation termination. Transient expression assays showed that the newly identified arrest-causing uORFs exerted a strong sequence-dependent repressive effect on the expression of the downstream reporter gene in A. thaliana protoplasts. These results suggest that the peptide sequences of the three uORFs identified in this study cause ribosomal arrest in the uORFs, thereby repressing the expression of proteins encoded by the main ORFs.
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