ArticleJournal of experimental botany2017
Measuring CO2 and HCO3- permeabilities of isolated chloroplasts using a MIMS-18O approach.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Lumen alkalization under elevated bicarbonate concentration outside isolated thylakoids of higher plants.Photosynthesis research · 2026Article
- Characterization and Computational Engineering of Structural Elements Controlling Gas Permeability in PIP2;1 Aquaporins.Journal of computational chemistry · 2026Article
- Heterologous expression in E. coli reveals that bicarbonate transporter BicA2 mediates carbon uptake in marine Prochlorococcus spp.The ISME journal · 2026Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Engineering the cyanobacterial ATP-driven BCT1 bicarbonate transporter for functional targeting to C3 plant chloroplasts.Journal of experimental botany · 2024Article
- Cooperation of an external carbonic anhydrase and HCO3- transporter supports underwater photosynthesis in submerged leaves of the amphibious plant Hygrophila difformis.Annals of botany · 2024Article
- The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration.Quantitative plant biology · 2024Article
- The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions inPlants (Basel, Switzerland) · 2022Article
- New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.Plant physiology · 2022Review
- Mesophyll conductance is unaffected by expression of Arabidopsis PIP1 aquaporins in the plasmalemma of Nicotiana.Journal of experimental botany · 2022Article
- Mitochondrial carbonic anhydrases are needed for optimal photosynthesis at low CO2 levels in Chlamydomonas.Plant physiology · 2021Article
- Absence of carbonic anhydrase in chloroplasts affects CProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns.Frontiers in plant science · 2021Review
- Membrane Inlet Mass Spectrometry at the Crossroads of Photosynthesis, Biofuel, and Climate Research.Plant physiology · 2020Article
- Recent advances in understanding and improving photosynthesis.Faculty reviews · 2020Review
- Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.Frontiers in plant science · 2020Article
- How protein - protein interactions contribute to pyrenoid formation in Chlamydomonas.Journal of experimental botany · 2019Article
- Computational modelling predicts substantial carbon assimilation gains for C3 plants with a single-celled C4 biochemical pump.PLoS computational biology · 2019Article
- Transport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead.International journal of molecular sciences · 2018Review
- Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To support photosynthetic CO2 fixation by Rubisco, the chloroplast must be fed with inorganic carbon in the form of CO2 or bicarbonate. However, the mechanisms allowing the rapid passage of this gas and this charged molecule through the bounding membranes of the chloroplast envelope are not yet completely elucidated. We describe here a method allowing us to measure the permeability of these two molecules through the chloroplast envelope using a membrane inlet mass spectrometer and 18O-labelled inorganic carbon. We established that the internal stromal carbonic anhydrase activity is not limiting for this technique, and precisely measured the chloroplast surface area and permeability values for CO2 and bicarbonate. This was performed on chloroplasts from several plant species, with values ranging from 2.3 × 10-4 m s-1 to 8 × 10-4 m s-1 permeability for CO2 and 1 × 10-8 m s-1 for bicarbonate. We were able to apply our method to chloroplasts from an Arabidopsis aquaporin mutant, and this showed that CO2 permeability was reduced 50% in the mutant compared with the wild-type reference.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.