ArticleBiophysical journal2017
Characterizing Intracellular Ice Formation of Lymphoblasts Using Low-Temperature Raman Spectroscopy.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Natural killer cell cryopreservation: Influence of composition, temperature, and granules on post-thaw survival and function.Molecular therapy. Advances · 2026Article
- Automated image analysis for intracellular ice detection in monolayers by freeze-substitution.Npj imaging · 2026Article
- Raman spectroscopic analysis of intracellular ice-induced degradation of mesenchymal stromal cells.Biophysical journal · 2026Article
- Mechanistic insights into how water-network disorder determines the vitrification concentration in cryopreservation solutions.Cell reports. Physical science · 2026Article
- DMSO-free cryopreservation of hiPSC-derived cardiomyocytes: low temperature characterization and protocol development.Stem cell research & therapy · 2025Article
- The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes.Frontiers in bioengineering and biotechnology · 2024Article
- Characterizing cellular membrane partitioning of DMSO using low-temperature Raman spectroscopy.Frontiers in molecular biosciences · 2023Article
- Peptide-DNA origami as a cryoprotectant for cell preservation.Science advances · 2022Article
- Winter is coming: the future of cryopreservation.BMC biology · 2021Review
- Non-Uniform Distribution of Cryoprotecting Agents in Rice Culture Cells Measured by CARS Microscopy.Plants (Basel, Switzerland) · 2021Article
- Preserving the Female Genome in Trehalose Glass at Supra-Zero Temperatures: The Relationship Between Moisture Content and DNA Damage in Feline Germinal Vesicles.Cellular and molecular bioengineering · 2021Article
- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cryoprotective enhancing effect of very low concentration of trehalose on the functions of primary rat hepatocytes.Regenerative therapy · 2020Article
- Understanding the freezing responses of T cells and other subsets of human peripheral blood mononuclear cells using DSMO-free cryoprotectants.Cytotherapy · 2020Article
- Article
- Cryopreservation of Human iPS Cell Aggregates in a DMSO-Free Solution-An Optimization and Comparative Study.Frontiers in bioengineering and biotechnology · 2020Article
- Interfacial Interactions of Sucrose during Cryopreservation Detected by Raman Spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Characterizing modes of action and interaction for multicomponent osmolyte solutions on Jurkat cells.Biotechnology and bioengineering · 2019Article
- Article
- Characterizing the "sweet spot" for the preservation of a T-cell line using osmolytes.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Raman microspectroscopy was used to quantify freezing response of cells to various cooling rates and solution compositions. The distribution pattern of cytochrome c in individual cells was used as a measure of cell viability in the frozen state and this metric agreed well with the population-averaged viability and trypan blue staining experiments. Raman imaging of cells demonstrated that intracellular ice formation (IIF) was common and did not necessarily result in cell death. The amount of intracellular ice as well as ice crystal size played a role in determining whether or not ice inside the cell was a lethal event. Intracellular ice crystals were colocated to the sections of cell membrane in close proximity to extracellular ice. Increasing the distance between extracellular ice and cell membrane decreased the incidence of IIF. Reducing the effective stiffness of the cell membrane by disrupting the actin cytoskeleton using cytochalasin D increased the amount of IIF. Strong intracellular osmotic gradients were observed when IIF was present. These observations support the hypothesis that interactions between the cell membrane and extracellular ice result in IIF. Raman spectromicroscopy provides a powerful tool for observing IIF and understanding its role in cell death during freezing, and enables the development, to our knowledge, of new and improved cell preservation protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.