ArticlePloS one2017
De novo transcriptome of the mayfly Cloeon viridulum and transcriptional signatures of Prometabola.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Taxonomic insights into the diversity ofZooKeys · 2026Article
- First report of mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2026Article
- Mayfly developmental atlas: developmental temporal expression atlas of the mayfly, Ephemera vulgata, reveals short germ-specific hox gene activation.BMC genomics · 2024Article
- Characterizing Hox genes in mayflies (Ephemeroptera), with Hexagenia limbata as a new mayfly model.EvoDevo · 2022Article
- Molecular mechanisms underlying metamorphosis in the most-ancestral winged insect.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Review of the GenusInsects · 2021Article
- Mayfly metamorphosis: Adult winged insects that molt.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Regulation of metamorphosis in neopteran insects is conserved in the paleopteranProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Spatial and morphological reorganization of endosymbiosis during metamorphosis accommodates adult metabolic requirements in a weevil.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The innovation of the final moult and the origin of insect metamorphosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).Scientific reports · 2019Article
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5 authors.
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Abstract
Mayflies (Ephemeroptera) display many primitive characters and a unique type of metamorphosis (Prometabola). However, information on the genomes and transcriptomes of this insect group is limited. The RNA sequencing study presented here generated the first de novo transcriptome assembly of Cloeon viridulum (Ephemeroptera: Baetidae), and compared gene expression signatures among the young larva (YL), mature larva (ML), subimago (SI), and imago (IM) stages of this mayfly. The transcriptome, based on 88 Gb of sequence data, comprised a set of 81,185 high quality transcripts. The number of differentially expressed genes (DEGs) in YL vs. ML, ML vs. SI, and SI vs. IM, was 4,825, 1,584, and 1,278, respectively, according to the reads per kilobase of transcript per million mapped reads analysis, assuming a false discovery rate <0.05 and a fold change >2. Gene enrichment analysis revealed that these DEGs were enriched in the "chitin metabolic process", "germ cell development", "steroid hormone biosynthesis", and "cutin, suberine, and wax biosynthesis" pathways. Finally, the expression pattern of a selected group of candidate signature genes for Prometabola, including vestigial, methoprene-tolerant, wingless, and broad-complex were confirmed by quantitative real time-PCR analysis. The Q-PCR analysis of larval, subimaginal, and imaginal stages of C. viridulum suggests that the development of mayflies more closely resembles hemimetamorphosis than holometamorphosis.
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