SynthesisFrontiers in cellular and infection microbiology2017
Reducing Viability Bias in Analysis of Gut Microbiota in Preterm Infants at Risk of NEC and Sepsis.
Synthesis in Frontiers in cellular and infection microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Bifidobacterium and Lactobacillus for preventing necrotizing enterocolitis in very-low-birth-weight preterm infants: a systematic review and meta-analysis.World journal of pediatrics : WJP · 2020Pooled it
- Multi-dimensional dynamic assessment revealed the antibiotic-induced pseudo-germ-free model in SD rats stabilized within 4 days.Frontiers in microbiology · 2026Article
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- Illumina Sequencing in Conjunction with Propidium Monoazide to Identify Live Bacteria After Antiseptic Treatment in a Complex Oral Biofilm: A Study Using an Ex Vivo Supragingival Biofilm Model.Antibiotics (Basel, Switzerland) · 2024Article
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- Built environment microbiomes transition from outdoor to human-associated communities after construction and commissioning.Scientific reports · 2023Article
- Intravenous antibiotics in preterm infants have a negative effect upon microbiome development throughout preterm life.Gut pathogens · 2023Article
- Early gut microbiota in very low and extremely low birth weight preterm infants with feeding intolerance: a prospective case-control study.Journal of microbiology (Seoul, Korea) · 2022Observational
- Cryopreservation of stool samples altered the microbial viability quantitively and compositionally.Archives of microbiology · 2022Article
- Optimisation and Application of a Novel Method to Identify Bacteriophages in Maternal Milk and Infant Stool Identifies Host-Phage Communities Within Preterm Infant Gut.Frontiers in pediatrics · 2022Article
- An Assay Combining Droplet Digital PCR With Propidium Monoazide Treatment for the Accurate Detection of Live Cells ofFrontiers in microbiology · 2022Article
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- Dead or alive: microbial viability treatment reveals both active and inactive bacterial constituents in the fish gut microbiota.Journal of applied microbiology · 2021Article
- Detection of viable and total fungal community in zaopei of Chinese strong-flavor baijiu using PMA combined with qPCR and HTS based on ITS2 region.BMC microbiology · 2021Article
- Methods for exploring the faecal microbiome of premature infants: a review.Maternal health, neonatology and perinatology · 2021Review
- Impact of Early Empiric Antibiotic Regimens on the Gut Microbiota in Very Low Birth Weight Preterm Infants: An Observational Study.Frontiers in pediatrics · 2021Article
- Free DNA and Metagenomics Analyses: Evaluation of Free DNA Inactivation Protocols for Shotgun Metagenomics Analysis of Human Biological Matrices.Frontiers in microbiology · 2021Article
- Poultry feeds carry diverse microbial communities that influence chicken intestinal microbiota colonisation and maturation.AMB Express · 2020Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Necrotising enterocolitis (NEC) and sepsis are serious diseases of preterm infants that can result in feeding intolerance, the need for bowel resection, impaired physiological and neurological development, and high mortality rates. Neonatal healthcare improvements have allowed greater survival rates in preterm infants leading to increased numbers at risk of developing NEC and sepsis. Gut bacteria play a role in protection from or propensity to these conditions and have therefore, been studied extensively using targeted 16S rRNA gene sequencing methods. However, exact epidemiology of these conditions remain unknown and the role of the gut microbiota in NEC remains enigmatic. Many studies have confounding variables such as differing clinical intervention strategies or major methodological issues such as the inability of 16S rRNA gene sequencing methods to determine viable from non-viable taxa. Identification of viable community members is important to identify links between the microbiota and disease in the highly unstable preterm infant gut. This is especially important as remnant DNA is robust and persists in the sampling environment following cell death. Chelation of such DNA prevents downstream amplification and inclusion in microbiota characterisation. This study validates use of propidium monoazide (PMA), a DNA chelating agent that is excluded by an undamaged bacterial membrane, to reduce bias associated with 16S rRNA gene analysis of clinical stool samples. We aim to improve identification of the viable microbiota in order to increase the accuracy of clinical inferences made regarding the impact of the preterm gut microbiota on health and disease. Gut microbiota analysis was completed on stools from matched twins (
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