ArticleJournal of investigative medicine : the official publication of the American Federation for Clinical Research2017
Role of autophagy in tumor necrosis factor-α-induced apoptosis of osteoblast cells.
Article in Journal of investigative medicine : the official publication of the American Federation for Clinical Research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- The association between irritable bowel syndrome and osteoporosis: a systematic review and meta-analysis.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2020Pooled it
- Crosstalk between inflammation and autophagy in CeD organoids.Scientific reports · 2026Article
- Non-damaging laser treatment with electroretinography-based thermal dosimetry activates hormetic heat response in pig retinal pigment epithelium.Nature communications · 2025Article
- Trehalose Rescues Postmenopausal Osteoporosis Induced by Ovariectomy through Alleviating Osteoblast Pyroptosis via Promoting Autophagy.Biomedicines · 2024Article
- Fibroblast growth factor 23 regulates hypoxia‑induced osteoblast apoptosis through the autophagy‑signaling pathway.Molecular medicine reports · 2023Article
- Carboxymethyl chitosan regulates oxidative stress and decreases the expression levels of tumor necrosis factor α in macrophages induced by wear particles.Cytotechnology · 2023Article
- Suffruticosol A elevates osteoblast differentiation targeting BMP2-Smad/1/5/8-RUNX2 in pre-osteoblasts.BioFactors (Oxford, England) · 2023Article
- AMPK/mTOR Pathway Is Involved in Autophagy Induced by Magnesium-Incorporated TiOJournal of functional biomaterials · 2022Article
- Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes.Journal of orthopaedic surgery and research · 2022Article
- Role of the major histocompatibility complex class II protein presentation pathway in bone immunity imbalance in postmenopausal osteoporosis.Frontiers in endocrinology · 2022Review
- Inhibition of TNF-Applied bionics and biomechanics · 2022Article
- Review
- Overexpression of TNFα induces senescence, autophagy and mitochondrial dysfunctions in melanoma cells.BMC cancer · 2021Article
- Berberine exerts a protective effect on rats with polycystic ovary syndrome by inhibiting the inflammatory response and cell apoptosis.Reproductive biology and endocrinology : RB&E · 2021Article
- T-Cell Mediated Inflammation in Postmenopausal Osteoporosis.Frontiers in immunology · 2021Review
- Osteoimmunology: The Regulatory Roles of T Lymphocytes in Osteoporosis.Frontiers in endocrinology · 2020Review
- Effect ofOncoTargets and therapy · 2020Article
- Caveolin as a Universal Target in Dermatology.International journal of molecular sciences · 2019Review
- Osthole improves therapy for osteoporosis through increasing autophagy of mesenchymal stem cells.Experimental animals · 2019Article
- Loading necrostatin-1 composite bone cement inhibits necroptosis of bone tissue in rabbit.Regenerative biomaterials · 2019Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study is to investigate the role of tumor necrosis factor-α (TNF-α) in apoptosis and autophagy of mouse osteoblast MC3T3-E1 cells, as well as the crosstalk between autophagy and apoptosis. Mouse osteoblast MC3T3-E1 cells were cultured in vitro and treated with 5-fluorouracil (5-FU), rapamycin, 3-methyl adenine (3-MA) and TNF-α either alone or in combination, respectively. MTT assays were used to monitor the cell viability upon different treatments. Annexin-V-FITC/propidium iodide (PI) staining was used to detect the apoptotic rate of osteoblasts. Autophagic structure and apoptotic bodies were visualized by transmission electron microscopy (TEM). Western blot analysis was performed to detect the autophagic marker LC3-II/I, p62 and apoptotic marker cleaved caspase-3. TNF-α inhibits MC3T3-E1 cell viability in a dose-dependent and time-dependent manner. Annexin-V-FITC/PI staining, coupled with TEM, showed that TNF-α induced cell apoptosis and autophagy in MC3T3-E1 cells. The autophagy inducer rapamycin ameliorated TNF-α-induced apoptosis. In contrast, 3-MA, which is an autophagy inhibitor, caused an exaggerated induction of TNF-α-induced apoptosis. TNF-α upregulated autophagy marker LC3-II/I, but downregulated p62 in osteoblasts. Combined treatment of rapamycin and TNF-α further exaggerated this effect, whereas co-treatment of 3-MA and TNF-α decreased LC3-II/I, but increased p62 compared with TNF-α alone. In addition, TNF-α caused an induction of apoptotic marker cleaved caspase-3. TNF-α-mediated induction of cleaved caspase-3 was downregulated by rapamycin, but upregulated by 3-MA, respectively. TNF-α induced both autophagy and apoptosis in osteoblasts, and upregulated autophagy protects the cell by reducing TNF-α-induced apoptosis.
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