Evidence map›Paper›PMID 28634192›Full record

ArticleJournal of lipid research2017

A new enzyme-linked immunosorbent assay system for human serum hepatic triglyceride lipase.

Kazuya Miyashita, Katsuyuki Nakajima, Isamu Fukamachi, Yuji Muraba, Takafumi Koga, Yohnosuke Shimomura, Tetsuyo Machida, Masami Murakami, Junji Kobayashi

Open access · hybridAbstract read
In one paragraph

Article in Journal of lipid research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Kazuya MiyashitaImmuno-Biological Laboratories Co., Ltd., Fujioka, Gunma, Japan.
Katsuyuki NakajimaDepartment of General Medicine, Kanazawa Medical University, Ishikawa, Japan; Hidaka Hospital, Takasaki, Japan.
Isamu FukamachiImmuno-Biological Laboratories Co., Ltd., Fujioka, Gunma, Japan.
Yuji MurabaHidaka Hospital, Takasaki, Japan.
Takafumi KogaHidaka Hospital, Takasaki, Japan.
Yohnosuke ShimomuraHidaka Hospital, Takasaki, Japan.
Tetsuyo MachidaDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Masami MurakamiDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Junji KobayashiDepartment of General Medicine, Kanazawa Medical University, Ishikawa, Japan. Electronic address: mary@kanazawa-med.ac.jp.
Hidaka Hospital · JPGunma University · JPMedical & Biological Laboratories (Japan) · JPKanazawa Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is no established method for measuring human hepatic triglyceride (TG) lipase (HTGL) concentration in serum. In this study, we developed new monoclonal Abs (MoAbs) (9A1 mouse MoAb and 141A1 rat MoAb) that react with HTGL both in serum and in postheparin plasma (PHP) and established a novel ELISA system for measuring serum HTGL and PHP-HTGL concentrations. To confirm the specificity of MoAbs, we performed immunoprecipitation-immunoblotting analysis. Both 9A1 mouse MoAb and 141A1 rat MoAb were able to immunoprecipitate not only recombinant HTGL and PHP-HTGL but also serum HTGL, demonstrating that HTGL exists in serum obtained without heparin injection. This method yielded intra- and interassay coefficients of variation of <6% and showed no cross-reactivity with LPL or endothelial lipase. In clinical analysis on 42 male subjects with coronary artery disease, there were strong positive correlations of serum HTGL concentration to PHP-HTGL concentration (

Indexed as

Antibodies, MonoclonalAntibody SpecificityBlood Chemical AnalysisEnzyme-Linked Immunosorbent AssayHumansLipaseLiverAntibodies, MonoclonalLipaselipase/hepaticlipase/lipoproteinlipoprotein/metabolism

Identifiers

PMID28634192
PMCPMC5538281
OpenAlexW2635596260

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.