ArticleDiagnostic microbiology and infectious disease2017
Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America.
Article in Diagnostic microbiology and infectious disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- CRISPR-Cas13a SHERLOCK assay for rapid and sensitive detection of chikungunya virus.Microbiology spectrum · 2026Article
- Chikungunya in 2025: Comprehensive Insights into Virology, Diagnostics, Vaccines, and Antiviral Therapies.Viruses · 2026Review
- In Vitro Evaluation of the Antiviral Effect ofViruses · 2025Article
- Predictability of infectious disease outbreak severity: Chikungunya as a case study.Science advances · 2025Article
- Development of an affordable multiplex quantitative RT-PCR assay for early detection and surveillance of Dengue, Chikungunya, and co-infections from clinical samples in resource-limited settings.PLoS neglected tropical diseases · 2025Article
- Chikungunya and Dengue Encephalitis: A Critical Narrative Review.Annals of Indian Academy of Neurology · 2025Article
- Pathogenesis and clinical management of arboviral diseases.World journal of virology · 2025Review
- In Vitro Evaluation of the Anti-Chikungunya Virus Activity of an Active Fraction Obtained fromViruses · 2024Article
- Pathogenicity and virulence of chikungunya virus.Virulence · 2024Review
- Analytical and Clinical Evaluation of a TaqMan Real-Time PCR Assay for the Detection of Chikungunya Virus.Microbiology spectrum · 2023Article
- Chikungunya virus infection induces ultrastructural changes and impaired neuronal differentiation of human neurospheres.Frontiers in microbiology · 2023Article
- Congenital chikungunya in a neonate with early-onset sepsis and petechiae: An unusual case report.Clinical case reports · 2023Article
- Understanding the Biology and Immune Pathogenesis of Chikungunya Virus Infection for Diagnostic and Vaccine Development.Viruses · 2022Review
- Neurologic Manifestations of the World Health Organization's List of Pandemic and Epidemic Diseases.Frontiers in neurology · 2021Review
- Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.PLoS neglected tropical diseases · 2019Article
- Cellular and Molecular Immune Response to Chikungunya Virus Infection.Frontiers in cellular and infection microbiology · 2018Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Chikungunya was introduced into the Americas in 2015 causing a pandemic across the continent. Testing during the acute phase of infection relies on qRT-PCR, but available assays have a number of limitations. A qRT-PCR assay specific to the chikungunya E1 gene was designed using sequence data from contemporary strains. A probit analysis established the 95% limit of detection as 19.6 copies per reaction. We compared the assay with a US Centers for Disease Control (CDC) chikungunya qRT-PCR as the reference standard. The assay had a sensitivity and specificity of 98.4% and 100% in 90 samples retrospectively collected in Guatemala. In a further 74 febrile samples prospectively collected in Ecuador and Guatemala the test had a sensitivity and specificity of 100% and 98.4%, respectively. Sequencing the nsp4 gene of the discordant positive sample indicated the presence of chikungunya RNA, and mismatches to the primer binding sites of the CDC assay.
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