ReviewThe review of diabetic studies : RDS2017
Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?
Review in The review of diabetic studies : RDS, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Comparison of islet isolation result and clinical applicability according to GMP-grade collagenase enzyme blend in adult porcine islet isolation and culture.Xenotransplantation · 2021Article
- Failure mode and effect analysis in human islet isolation: from the theoretical to the practical risk.Islets · 2021Article
- A Practical Guide to Rodent Islet Isolation and Assessment Revisited.Biological procedures online · 2021Review
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- Pancreatic islet transplantation: toward definitive treatment for diabetes mellitus.Global health & medicine · 2020Review
- Trisected pancreas model for testing tissue dissociation enzyme combinations: a novel methodology for improving human islet yield for clinical islet transplantation.Journal of diabetes and metabolic disorders · 2020Article
- Recombinant collagenase from Grimontia hollisae as a tissue dissociation enzyme for isolating primary cells.Scientific reports · 2020Article
- Article
- A modified culture medium for improved isolation of marine vibrios.MicrobiologyOpen · 2019Article
- Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation.Cell transplantation · 2019Article
- Effectiveness of different molecular forms of C. histolyticum class I collagenase to recover islets.Islets · 2017Article
- Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity.Cell transplantation · 2017Article
- Comparison of Islet Characterization from Use of Standard Crude Collagenase to GMP-Grade Collagenase Enzyme Blends in Preweaned Porcine Islet Isolations.Cell transplantationArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In comparison to procedures used for the separation of individual cell types from other organs, the process of human pancreatic islet isolation aims to digest the pancreatic exocrine matrix completely without dispersing the individual cells within the endocrine cell cluster. This objective is unique within the field of tissue separation, and outlines the challenge of islet isolation to balance two opposing priorities. Although significant progress has been made in the characterization and production of enzyme blends for islet isolation, there are still numerous areas which require improvement. The ultimate goal of enzyme production, namely the routine production of a consistent and standardized enzyme blend, has still not been realized. This seems to be mainly the result of a lack of detailed knowledge regarding the structure of the pancreatic extracellular matrix and the synergistic interplay between collagenase and different supplementary proteases during the degradation of the extracellular matrix. Furthermore, the activation of intrinsic proteolytic enzymes produced by the pancreatic acinar cells, also impacts on the chance of a successful outcome of human islet isolation. This overview discusses the challenges of pancreatic enzymatic digestion during human islet isolation, and outlines the developments in this field over the past 5 decades.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.