ArticlePloS one2017
Combining 'omics and microscopy to visualize interactions between the Asian citrus psyllid vector and the Huanglongbing pathogen Candidatus Liberibacter asiaticus in the insect gut.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 144 citations in OpenAlex.
- Protein kinase R-like ER kinase (PERK) is involved in the endoplasmic reticulum stress response of its psyllid vector toMicrobiology spectrum · 2026Article
- Recombinant cystatin ingestion byFrontiers in insect science · 2026Article
- Rewiring the proteome of the Euscelidius variegatus holobiont in response to Flavescence dorée phytoplasma.Scientific reports · 2025Article
- Enigmatic tubular ultrastructure in the bacterial defensive symbiont of the Asian citrus psyllid Diaphorina citri.Npj imaging · 2025Article
- Bacterial vector-borne plant diseases: global issues caused by three-way interactions.Crop health · 2025Review
- An insect virus differentially alters gene expression among life stages of an insect vector and enhances bacterial phytopathogen transmission.Journal of virology · 2025Article
- Ultrastructure of the bacteriome and bacterial symbionts in the Asian citrus psyllid,Microbiology spectrum · 2024Article
- Diaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing.GigaScience · 2024Article
- Detection ofFrontiers in plant science · 2024Article
- Effects of Candidatus Liberibacter asiaticus infection on metagenome of Diaphorina citri gut endosymbiont.Scientific data · 2023Article
- Spatial Distribution and Temporal Dynamics ofInternational journal of molecular sciences · 2023Article
- RNA interference-mediated knockdown of genes involved in sugar transport and metabolism disrupts psyllidFrontiers in insect science · 2023Article
- Differential gene expression of Asian citrus psyllids infected with 'Frontiers in plant science · 2023Article
- 16S rRNA Gene Sequencing of Six Psyllid Species of the Family Carsidaridae Identified Various Bacteria Including Symbiopectobacterium.Microbes and environments · 2023Article
- Sub-optimal temperatures lead to altered expression of stress-related genes and increased 'CFrontiers in insect science · 2023Article
- Potato psyllids mount distinct gut responses against two different 'Candidatus Liberibacter solanacearum' haplotypes.PloS one · 2023Article
- Microbiome of pear psyllids: A tale about closely related species sharing their endosymbionts.Environmental microbiology · 2022Article
- Wolbachia infection dynamics in a natural population of the pear psyllid Cacopsylla pyri (Hemiptera: Psylloidea) across its seasonal generations.Scientific reports · 2022Article
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2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Huanglongbing, or citrus greening disease, is an economically devastating bacterial disease of citrus. It is associated with infection by the gram-negative bacterium Candidatus Liberibacter asiaticus (CLas). CLas is transmitted by Diaphorina citri, the Asian citrus psyllid (ACP). For insect transmission to occur, CLas must be ingested during feeding on infected phloem sap and cross the gut barrier to gain entry into the insect vector. To investigate the effects of CLas exposure at the gut-pathogen interface, we performed RNAseq and mass spectrometry-based proteomics to analyze the transcriptome and proteome, respectively, of ACP gut tissue. CLas exposure resulted in changes in pathways involving the TCA cycle, iron metabolism, insecticide resistance and the insect's immune system. We identified 83 long non-coding RNAs that are responsive to CLas, two of which appear to be specific to the ACP. Proteomics analysis also enabled us to determine that Wolbachia, a symbiont of the ACP, undergoes proteome regulation when CLas is present. Fluorescent in situ hybridization (FISH) confirmed that Wolbachia and CLas inhabit the same ACP gut cells, but do not co-localize within those cells. Wolbachia cells are prevalent throughout the gut epithelial cell cytoplasm, and Wolbachia titer is more variable in the guts of CLas exposed insects. CLas is detected on the luminal membrane, in puncta within the gut epithelial cell cytoplasm, along actin filaments in the gut visceral muscles, and rarely, in association with gut cell nuclei. Our study provides a snapshot of how the psyllid gut copes with CLas exposure and provides information on pathways and proteins for targeted disruption of CLas-vector interactions at the gut interface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.