ArticleScientific reports2017
Overcoming evolved resistance to population-suppressing homing-based gene drives.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 178 citations in OpenAlex.
- Split suppression gene drives reveal a design tradeoff between inheritance and reproductive load inbioRxiv : the preprint server for biology · 2026Article
- Upper bound on the mutational burden imposed by a CRISPR-Cas9 gene-drive element.G3 (Bethesda, Md.) · 2026Article
- Engineering resilient gene drives for sustainable malaria control by predicting, testing and overcoming target site resistance in Anopheles gambiae.PLoS biology · 2026Article
- Integrating multiplexing into confineable gene drives effectively overrides resistance in Anopheles stephensi.Nature communications · 2026Article
- Advances in CRISPR gene drives for mosquito population control.Current opinion in microbiology · 2026Review
- Strategies to improve the efficiency of homing gene drives with multiplexed gRNAs.BMC biology · 2025Article
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- Experimental Test of Evolutionary Safety of a CRISPR-Cas9 Gene-Drive Element.bioRxiv : the preprint server for biology · 2025Article
- Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi.Parasites & vectors · 2025Article
- A Y chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae.Nature communications · 2025Article
- A model-informed target product profile for population modification gene drives for malaria control.PLOS global public health · 2025Article
- Cleave and Rescue gamete killers create conditions for gene drive in plants.Nature plants · 2024Article
- MGSurvE: A framework to optimize trap placement for genetic surveillance of mosquito populations.PLoS computational biology · 2024Article
- Cleave and Rescue gamete killers create conditions for gene drive in plants.bioRxiv : the preprint server for biology · 2024Article
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Daisy-chain gene drives: The role of low cut-rate, resistance mutations, and maternal deposition.PLoS genetics · 2022Article
- A theory of resistance to multiplexed gene drive demonstrates the significant role of weakly deleterious natural genetic variation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Population replacement gene drive characteristics for malaria elimination in a range of seasonal transmission settings: a modelling study.Malaria journal · 2022Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The recent development of a CRISPR-Cas9-based homing system for the suppression of Anopheles gambiae is encouraging; however, with current designs, the slow emergence of homing-resistant alleles is expected to result in suppressed populations rapidly rebounding, as homing-resistant alleles have a significant fitness advantage over functional, population-suppressing homing alleles. To explore this concern, we develop a mathematical model to estimate tolerable rates of homing-resistant allele generation to suppress a wild population of a given size. Our results suggest that, to achieve meaningful population suppression, tolerable rates of resistance allele generation are orders of magnitude smaller than those observed for current designs for CRISPR-Cas9-based homing systems. To remedy this, we theoretically explore a homing system architecture in which guide RNAs (gRNAs) are multiplexed, increasing the effective homing rate and decreasing the effective resistant allele generation rate. Modeling results suggest that the size of the population that can be suppressed increases exponentially with the number of multiplexed gRNAs and that, with four multiplexed gRNAs, a mosquito species could potentially be suppressed on a continental scale. We also demonstrate successful proof-of-principle use of multiplexed ribozyme flanked gRNAs to induce mutations in vivo in Drosophila melanogaster - a strategy that could readily be adapted to engineer stable, homing-based drives in relevant organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.