Evidence map›Paper›PMID 28629175›Full record

ArticleInternational journal of molecular sciences2017

The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young's Modulus of Single Cells.

Ke Wang, Yang Zhao, Deyong Chen, Chengjun Huang, Beiyuan Fan, Rong Long, Chia-Hsun Hsieh, Junbo Wang, Min-Hsien Wu, Jian Chen

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ke WangState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. wangke2014@mails.ucas.ac.cn.
Yang ZhaoInstitute of Microelectronics of Chinese Academy of Sciences, Beijing 100029, China. zhaoyang@ime.ac.cn.
Deyong ChenState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. dychen@mail.ie.ac.cn.
Chengjun HuangInstitute of Microelectronics of Chinese Academy of Sciences, Beijing 100029, China. huangchengjun@ime.ac.cn.
Beiyuan FanState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. fanbeiyuan@ucas.ac.cn.
Rong LongDepartment of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA. Rong.Long@colorado.edu.
Chia-Hsun HsiehDivision of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33302, Taiwan. wisdom5000@gmail.com.
Junbo WangState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. jbwang@mail.ie.ac.cn.
Min-Hsien WuDivision of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33302, Taiwan. mhwu@mail.cgu.edu.tw.
Jian ChenState Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. chenjian@mail.ie.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This paper presents the instrumentation of a microfluidic analyzer enabling the characterization of single-cell biophysical properties, which includes seven key components: a microfluidic module, a pressure module, an imaging module, an impedance module, two LabVIEW platforms for instrument operation and raw data processing, respectively, and a Python code for data translation. Under the control of the LabVIEW platform for instrument operation, the pressure module flushes single cells into the microfluidic module with raw biophysical parameters sampled by the imaging and impedance modules and processed by the LabVIEW platform for raw data processing, which were further translated into intrinsic cellular biophysical parameters using the code developed in Python. Based on this system, specific membrane capacitance, cytoplasm conductivity, and instantaneous Young's modulus of three cell types were quantified as 2.76 ± 0.57 μF/cm², 1.00 ± 0.14 S/m, and 3.79 ± 1.11 kPa for A549 cells (

Indexed as

Elastic ModulusElectric CapacitanceElectric ConductivityBiophysical PhenomenaBiosensing TechniquesCell MembraneCytoplasmElectric ImpedanceElectronic Data ProcessingMicrofluidic Analytical TechniquesMicrofluidicsSingle-Cell Analysiscytoplasm conductivityinstantaneous Young’s modulusinstrumentationmicrofluidicssingle-cell analysisspecific membrane capacitance

Identifiers

PMID28629175
PMCPMC5485982

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.