ArticleBiotechnology and bioengineering2017
Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor.
Article in Biotechnology and bioengineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Advanced Extractables and Leachables Assessment of Microcarriers Used for Adherent Cell Cultures.Biotechnology journal · 2026Article
- Bioreactors expansion of human mesenchymal stromal cell therapies: platforms, parameters, challenges and opportunities.Journal, genetic engineering & biotechnology · 2026Review
- Edible Scaffolds for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- Emerging technologies towards extracellular vesicles large-scale production.Bioactive materials · 2025Review
- Expansion of induced pluripotent stem cells under consideration of bioengineering aspects: part 1.Applied microbiology and biotechnology · 2025Article
- Cell retention in scalable, perfusion-based mesenchymal stem cell expansion processes: a proof of concept.Frontiers in bioengineering and biotechnology · 2025Article
- Seed Train Optimization in Microcarrier-Based Cell Culture Post In Situ Cell Detachment through Scale-Down Hybrid Modeling.Bioengineering (Basel, Switzerland) · 2024Article
- A quality-by-design approach to improve process understanding and optimise the production and quality of CAR-T cells in automated stirred-tank bioreactors.Frontiers in immunology · 2024Article
- Miniaturization of hiPSC-derived 3D neural cultures in stirred-tank bioreactors for parallelized preclinical assessment of rAAV.Frontiers in bioengineering and biotechnology · 2024Article
- Enhanced burn wound healing by controlled-release 3D ADMSC-derived exosome-loaded hyaluronan hydrogel.Regenerative biomaterials · 2024Article
- Scalable manufacturing of gene-modified human mesenchymal stromal cells with microcarriers in spinner flasks.Applied microbiology and biotechnology · 2023Article
- Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing.Biotechnology letters · 2023Article
- Mesenchymal and induced pluripotent stem cell-based therapeutics: a comparison.Applied microbiology and biotechnology · 2023Review
- Needle to needle robot-assisted manufacture of cell therapy products.Bioengineering & translational medicine · 2022Article
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- Comparison of 2,3,5,4'-tetrahydroxystilbene-2-O-b-D-glucoside-induced proliferation and differentiation of dental pulp stem cells in 2D and 3D culture systems-gene analysis.Journal of dental sciences · 2022Article
- Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications.Bioengineering (Basel, Switzerland) · 2021Review
- Design and development of a new ambr250® bioreactor vessel for improved cell and gene therapy applications.Biotechnology letters · 2021Article
- Expansion of human mesenchymal stem/stromal cells on temporary liquid microcarriers.Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2021Article
- Numerical Methods for the Design and Description of In Vitro Expansion Processes of Human Mesenchymal Stem Cells.Advances in biochemical engineering/biotechnology · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbioreactors play a critical role in process development as they reduce reagent requirements and can facilitate high-throughput screening of process parameters and culture conditions. Here, we have demonstrated and explained in detail, for the first time, the amenability of the automated ambr15 cell culture microbioreactor system for the development of scalable adherent human mesenchymal multipotent stromal/stem cell (hMSC) microcarrier culture processes. This was achieved by first improving suspension and mixing of the microcarriers and then improving cell attachment thereby reducing the initial growth lag phase. The latter was achieved by using only 50% of the final working volume of medium for the first 24 h and using an intermittent agitation strategy. These changes resulted in >150% increase in viable cell density after 24 h compared to the original process (no agitation for 24 h and 100% working volume). Using the same methodology as in the ambr15, similar improvements were obtained with larger scale spinner flask studies. Finally, this improved bioprocess methodology based on a serum-based medium was applied to a serum-free process in the ambr15, resulting in >250% increase in yield compared to the serum-based process. At both scales, the agitation used during culture was the minimum required for microcarrier suspension, N
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