Evidence map›Paper›PMID 28626716›Full record

ArticleBiotechnology reports (Amsterdam, Netherlands)2015

Highly sensitive detection of influenza virus in saliva by real-time PCR method using sugar chain-immobilized gold nanoparticles; application to clinical studies.

Yasuo Suda, Mami Nagatomo, Risa Yokoyama, Mami Ohzono, Kazue Aoyama, Xu Zhang, Kazuhiko Nakajima, Naoki Murakami, Tadashi Shinoda, Tatsuhiko Hirota and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology reports (Amsterdam, Netherlands), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Functional role of surface layer proteins ofBioscience of microbiota, food and health · 2021
    Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Yasuo SudaDepartment of Chemistry, Biotechnology and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
Mami NagatomoSUDx-Biotec Corporation, 1-42-1 Shiroyama, Kagoshima, Kagoshima 890-0013, Japan.
Risa YokoyamaSUDx-Biotec Corporation, 1-42-1 Shiroyama, Kagoshima, Kagoshima 890-0013, Japan.
Mami OhzonoSUDx-Biotec Corporation, 1-42-1 Shiroyama, Kagoshima, Kagoshima 890-0013, Japan.
Kazue AoyamaSUDx-Biotec Corporation, 1-42-1 Shiroyama, Kagoshima, Kagoshima 890-0013, Japan.
Xu ZhangSUDx-Biotec Corporation, 1-42-1 Shiroyama, Kagoshima, Kagoshima 890-0013, Japan.
Kazuhiko NakajimaDepartment of Infection Control and Prevention, Hyogo College of Medicine, 1-1 Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
Naoki MurakamiMurakami Clinic for Children, 9-1 Dairyu, Kagoshima, Kagoshima 892-0805, Japan.
Tadashi ShinodaResearch and Development Planning Department, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-0206, Japan.
Tatsuhiko HirotaMicrobiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-0206, Japan.
Sae YanagiharaMicrobiology and Fermentation Laboratory, Calpis Co. Ltd., 5-11-10 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-0206, Japan.
Jun-Ichiro NishiDepartment of Microbiology, Graduate School of Dental and Medical Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-0075, Japan.
Lintec Corporation (Japan) · JPKagoshima University · JPHyogo Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A highly sensitive and convenient method for detecting influenza virus was developed using modified end-point melt curve analysis of a RT-qPCR SYBR Green method and influenza virus-binding sugar chain-immobilized gold-nanoparticles (SGNP). Because SGNPs capture influenza viruses, the virus-SGNP complex was separated easily by centrifugation. Viral RNA was detected at very low concentrations, suggesting that SGNP increased sensitivity compared with standard methods. This method was applied to clinical studies. Influenza viruses were detected in saliva of patients or inpatients who had been considered influenza-free by a rapid diagnostic assay of nasal swabs. Furthermore, the method was applied to a human trial of prophylactic anti-influenza properties of yogurt containing

Indexed as

Detecting influenza virusHA, hemagglutininLactobacillus acidophilus L-92RT-qPCR-SYBR, reverse transcription real-time quantitative PCR SYBR Green methodSC, Sugar ChipSGNP, sugar chain-immobilized gold nanoparticlesSPR, surface plasmon resonanceSugar chain-immobilized gold-nanoparticle

Identifiers

PMID28626716
PMCPMC5466045
OpenAlexW244264096

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.