Evidence map›Paper›PMID 28625991›Full record

ArticleApplied and environmental microbiology2017

Different Amplicon Targets for Sequencing-Based Studies of Fungal Diversity.

Francesca De Filippis, Manolo Laiola, Giuseppe Blaiotta, Danilo Ercolini

Abstract readComparative Study
In one paragraph

Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
65citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

65 citing papers in PubMed, 1 synthesis or guideline pooled it.

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5 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Francesca De FilippisDepartment of Agricultural Sciences, University of Naples Federico II, Naples, Italy.ORCID http://orcid.org/0000-0002-3474-2884
Manolo LaiolaDepartment of Agricultural Sciences, University of Naples Federico II, Naples, Italy.
Giuseppe BlaiottaDepartment of Agricultural Sciences, University of Naples Federico II, Naples, Italy.
Danilo ErcoliniDepartment of Agricultural Sciences, University of Naples Federico II, Naples, Italy ercolini@unina.it.ORCID http://orcid.org/0000-0003-3061-9560

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Target-gene amplicon sequencing is the most exploited high-throughput sequencing application in microbial ecology. The targets are taxonomically relevant genes, with 16S rRNA being the gold standard for bacteria. As for fungi, the most commonly used target is the internal transcribed spacer (ITS). However, the uneven ITS length among species may promote preferential amplification and sequencing and incorrect estimation of their abundance. Therefore, the use of different targets is desirable. We evaluated the use of three different target amplicons for the characterization of fungal diversity. After an

Indexed as

BiodiversityDNA, FungalDNA, IntergenicDNA PrimersFungiHigh-Throughput Nucleotide SequencingMycological Typing TechniquesRNA, Ribosomal, 18SDNA, FungalDNA, IntergenicDNA PrimersRNA, Ribosomal, 18Samplicon sequencingfungal diversityhigh-throughput sequencingmicrobial ecologyprimer bias

Identifiers

PMID28625991
PMCPMC5561290

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.