ReviewCurrent opinion in microbiology2017
Toward a genetic tool development pipeline for host-associated bacteria.
Review in Current opinion in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Commensal bacteria inhibit viral infections via a tryptophan metabolite.Nature communications · 2026Article
- Oral Cavity Antibacterial Discovery Pipeline Driven by Advanced Analytical Techniques.Molecules (Basel, Switzerland) · 2026Review
- Anabaena-a promising chassis for space exploration.NPJ microgravity · 2026Review
- Microbiome engineering: unlocking therapeutic potential in inflammatory bowel disease.Frontiers in microbiology · 2025Review
- A Critical Review on Akkermansia muciniphila: Functional Mechanisms, Technological Challenges, and Safety Issues.Probiotics and antimicrobial proteins · 2024Review
- Microbiota metabolism of intestinal amino acids impacts host nutrient homeostasis and physiology.Cell host & microbe · 2024Article
- A Hitchhiker's guide to CRISPR editing tools in bacteria : CRISPR can help unlock the bacterial world, but technical and regulatory barriers persist.EMBO reports · 2024Article
- An adapted method for Cas9-mediated editing reveals the species-specific role of β-glucoside utilization driving competition betweenJournal of bacteriology · 2024Article
- An engineered bacterial symbiont allows noninvasive biosensing of the honey bee gut environment.PLoS biology · 2024Article
- A toolbox for genetic manipulation in intestinalSynthetic and systems biotechnology · 2024Article
- Advanced probiotics: bioengineering and their therapeutic application.Molecular biology reports · 2024Review
- Genetically Engineered Microorganisms and Their Impact on Human Health.International journal of clinical practice · 2024Review
- Genetic Engineering of Resident Bacteria in the Gut Microbiome.Journal of bacteriology · 2023Review
- The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae.ISME communications · 2023Article
- Future Modulation of Gut Microbiota: From Eubiotics to FMT, Engineered Bacteria, and Phage Therapy.Antibiotics (Basel, Switzerland) · 2023Review
- The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study ofbioRxiv : the preprint server for biology · 2023Article
- Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations.Frontiers in bioengineering and biotechnology · 2023Article
- Methods of DNA introduction for the engineering of commensal microbes.Engineering microbiology · 2022Review
- Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiome.Cell · 2022Article
- A quantitative model for metabolic intervention using gut microbes.Biotechnology progress · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Bacteria reside in externally accessible niches on and in multicellular organisms, often forming mutualistic relationships with their host. Recent studies have linked the composition of these microbial communities with alterations in the host's health, behavior, and development, yet the causative mediators of host-microbiota interactions remain poorly understood. Advances in understanding and engineering these interactions require the development of genetic tools to probe the molecular interactions driving the structure and function of microbial communities as well as their interactions with their host. This review discusses the current challenges to rendering culturable, non-model members of microbial communities genetically tractable - including overcoming barriers to DNA delivery, achieving predictable gene expression, and applying CRISPR-based tools - and details recent efforts to create generalized pipelines that simplify and expedite the tool-development process. We use the bacteria present in the human gastrointestinal tract as representative microbiota to illustrate some of the recent achievements and future opportunities for genetic tool development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.