ArticleMethods (San Diego, Calif.)2017
Analysis of mRNA deadenylation by multi-protein complexes.
Article in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed.
- Multivalent interactions with CCR4-NOT and PABPC1 determine mRNA repression efficiency by tristetraprolin.Nature communications · 2025Article
- Intracellular pathogen effector reprograms host gene expression by inhibiting mRNA decay.Nature communications · 2025Article
- MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA.Nature communications · 2025Article
- Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution.Nature structural & molecular biology · 2024Article
- Analysis of Human Endogenous mRNA Deadenylation Complexes by High-Resolution Gel Electrophoresis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A FRET-Based Assay and Computational Tools to Quantify Enzymatic Rates and Explore the Mechanisms of RNA Deadenylases in Heterogeneous Environments.Methods in molecular biology (Clifton, N.J.) · 2024Article
- In Vitro Reconstitution of the Drosophila melanogaster CCR4-NOT Complex to Assay Deadenylation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Activity.Methods in molecular biology (Clifton, N.J.) · 2024Article
- RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system.Nucleic acids research · 2023Article
- Regulation of the multisubunit CCR4-NOT deadenylase in the initiation of mRNA degradation.Current opinion in structural biology · 2022Review
- The PPR domain of mitochondrial RNA polymerase is an exoribonuclease required for mtDNA replication in Drosophila melanogaster.Nature cell biology · 2022Article
- RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation.Nature communications · 2021Article
- Poly(A) tail dynamics: Measuring polyadenylation, deadenylation and poly(A) tail length.Methods in enzymology · 2021Article
- Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation.Nature communications · 2019Article
- Importance of the Conserved Carboxyl-Terminal CNOT1 Binding Domain to Tristetraprolin ActivityMolecular and cellular biology · 2019Article
- Engineered viral RNA decay intermediates to assess XRN1-mediated decay.Methods (San Diego, Calif.) · 2019Article
- Article
- mRNA Deadenylation Is Coupled to Translation Rates by the Differential Activities of Ccr4-Not Nucleases.Molecular cell · 2018Article
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Authors and funding
4 authors.
Funding
Abstract
Poly(A) tails are found at the 3' end of almost every eukaryotic mRNA and are important for the stability of mRNAs and their translation into proteins. Thus, removal of the poly(A) tail, a process called deadenylation, is critical for regulation of gene expression. Most deadenylation enzymes are components of large multi-protein complexes. Here, we describe an in vitro deadenylation assay developed to study the exonucleolytic activities of the multi-protein Ccr4-Not and Pan2-Pan3 complexes. We discuss how this assay can be used with short synthetic RNAs, as well as longer RNA substrates generated using in vitro transcription. Importantly, quantitation of the reactions allows detailed analyses of deadenylation in the presence and absence of accessory factors, leading to new insights into targeted mRNA decay.
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