Article3 Biotech2017
Enzymatic saccharification of pretreated rice straw by cellulases from Aspergillus niger BK01.
Article in 3 Biotech, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Cellulase and β-glucosidase activity of crude enzyme extracted from fresh and expired soybean tempeh.Iranian journal of microbiology · 2026Article
- Fungal cellulases: a comprehensive review on production, innovations, and applications.Archives of microbiology · 2026Review
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- Article
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- Enhancing Antioxidants Extraction from Agro-Industrial By-Products by Enzymatic Treatment.Foods (Basel, Switzerland) · 2022Article
- Unique pool of carbohydrate-degrading enzymes in novel bacteria assembled from cow and buffalo rumen metagenomes.Applied microbiology and biotechnology · 2022Article
- Fermentation Quality and Microbial Community of Corn Stover or Rice Straw Silage Mixed with Soybean Curd Residue.Animals : an open access journal from MDPI · 2022Article
- Biological activity of chitosan inducing resistance efficiency of rice (Oryza sativa L.) after treatment with fungal based chitosan.Scientific reports · 2021Article
- Rice straw fermentation byJournal, genetic engineering & biotechnology · 2018Article
- Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkali-assisted acid pretreated rice straw was saccharified using cellulase from Aspergillus niger BK01. The cellulase production by the fungus was enhanced by parametric optimization using solid-state fermentation conditions. Maximum cellulase production (12.0 U/gds of carboxymethyl cellulase, CMCase) was achieved in 96 h, using 6.0% substrate concentration, 7.5% inoculum concentration, 1:2 solid to liquid ratio, at pH 5.5, and temperature 28 °C, by supplementation of the fermentation medium with 0.1% carboxymethylcellulose and 0.1% ammonium nitrate. Characterization of crude cellulases showed that highest CMCase activity was observed at pH 4.8 and temperature 40 °C. The CMCase was stable from pH 4.8-5.5 and at a temperature range of 35-50 °C. The pretreated biomass was subjected to hydrolysis with the fungal cellulases. The saccharification optimization studies showed that 2% (v/v) enzyme concentration and hydrolysis time of 2.5 h were optimum for maximum yield, i.e, 23.78% sugars and 35.96% saccharification value.
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Registered trials
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