ArticleScientific reports2017
A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Innervation of the anal canal revisited through micro-CT imaging.Journal of anatomy · 2026Article
- Thin-Adipose Compartment at the Colonic Mesentery-Perirenal Fat Interface: Histological and Three-Dimensional Morphological Studies.International journal of urology : official journal of the Japanese Urological Association · 2026Article
- Article
- 3D Imaging of Gene Expression Domains in the Mouse Heart.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The histone methyltransferase KMT2D is essential for embryo implantation via regulating precise differentiation of endometrial cells.Cell death discovery · 2024Article
- Development of a heat-stable alkaline phosphatase reporter system for cis-regulatory analysis and its application to 3D digital imaging of Xenopus embryonic tissues.Development, growth & differentiation · 2024Article
- Correlative microscopy and block-face imaging (CoMBI): a 3D imaging method with wide applicability in the field of biological science.Anatomical science international · 2023Review
- Regenerative Polarity of the Fin Ray in Zebrafish Caudal Fin and Related Tissue Formation on the Cut Surface.Journal of developmental biology · 2021Article
- A Non-Invasive Approach in the Assessment of Stress Phenomena and Impairment Values in Pea Seeds Caused by Pea Weevil.Plants (Basel, Switzerland) · 2021Article
- Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens.Scientific reports · 2021Article
- Computational analyses decipher the primordial folding coding the 3D structure of the beetle horn.Scientific reports · 2021Article
- Neural regulation in tooth regeneration of Ambystoma mexicanum.Scientific reports · 2020Article
- In Vivo Tracking of Tissue Engineered Constructs.Micromachines · 2019Review
- An enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction.BMC neuroscience · 2019Article
- On the Usage of Brain Atlases in Neuroimaging Research.Molecular imaging and biology · 2018Review
- Complex furrows in a 2D epithelial sheet code the 3D structure of a beetle horn.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the required system for our method is characterized by cost-effectiveness. Here, we demonstrated the utility of CoMBI using specimens ranging in size from several millimeters to several centimeters, i.e., mouse embryos, human brainstem samples, and stag beetle larvae, and present successful correlation between the 2D light microscopic images and 3D volume data in a single specimen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.