ArticleScientific reports2017
Multiplexed Spliced-Leader Sequencing: A high-throughput, selective method for RNA-seq in Trypanosomatids.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- SLRanger: an integrated approach for spliced leader detection and operon prediction using long RNA reads.Briefings in bioinformatics · 2025Article
- Bioengineered 3D microvessels and complementary animal models reveal mechanisms of Trypanosoma congolense sequestration.Communications biology · 2025Article
- Four layer multi-omics reveals molecular responses to aneuploidy in Leishmania.PLoS pathogens · 2022Article
- Transcriptomic profiling of Trypanosoma congolense mouthpart parasites from naturally infected flies.Parasites & vectors · 2022Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Central role of metabolism in Trypanosoma cruzi tropism and Chagas disease pathogenesis.Current opinion in microbiology · 2021Review
- SLIDR and SLOPPR: flexible identification of spliced leader trans-splicing and prediction of eukaryotic operons from RNA-Seq data.BMC bioinformatics · 2021Article
- Culture-free genome-wide locus sequence typing (GLST) provides new perspectives on Trypanosoma cruzi dispersal and infection complexity.PLoS genetics · 2020Article
- SLFinder, a pipeline for the novel identification of splice-leader sequences: a good enough solution for a complex problem.BMC bioinformatics · 2020Article
- Functional genomics in sand fly-derived Leishmania promastigotes.PLoS neglected tropical diseases · 2019Review
- In Search of Biomarkers for Pathogenesis and Control of Leishmaniasis by Global Analyses ofFrontiers in cellular and infection microbiology · 2018Review
- Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review.Iranian journal of parasitologyReview
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
High throughput sequencing techniques are poorly adapted for in vivo studies of parasites, which require prior in vitro culturing and purification. Trypanosomatids, a group of kinetoplastid protozoans, possess a distinctive feature in their transcriptional mechanism whereby a specific Spliced Leader (SL) sequence is added to the 5'end of each mRNA by trans-splicing. This allows to discriminate Trypansomatid RNA from mammalian RNA and forms the basis of our new multiplexed protocol for high-throughput, selective RNA-sequencing called SL-seq. We provided a proof-of-concept of SL-seq in Leishmania donovani, the main causative agent of visceral leishmaniasis in humans, and successfully applied the method to sequence Leishmania mRNA directly from infected macrophages and from highly diluted mixes with human RNA. mRNA profiles obtained with SL-seq corresponded largely to those obtained from conventional poly-A tail purification methods, indicating both enumerate the same mRNA pool. However, SL-seq offers additional advantages, including lower sequencing depth requirements, fast and simple library prep and high resolution splice site detection. SL-seq is therefore ideal for fast and massive parallel sequencing of parasite transcriptomes directly from host tissues. Since SLs are also present in Nematodes, Cnidaria and primitive chordates, this method could also have high potential for transcriptomics studies in other organisms.
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