ArticlePloS one2017
Large-scale modulation of reconstituted Min protein patterns and gradients by defined mutations in MinE's membrane targeting sequence.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design.Cell · 2024Article
- The mechanism of MinD stability modulation by MinE in Min protein dynamics.PLoS computational biology · 2023Article
- Increasing MinD's Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes.ACS synthetic biology · 2021Article
- Engineering spatiotemporal organization and dynamics in synthetic cells.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2021Review
- Probing Biomolecular Interactions by a Pattern-Forming Peptide-Conjugate Sensor.Bioconjugate chemistry · 2021Article
- C-terminal eYFP fusion impairsOpen biology · 2020Article
- Article
- The E. coli MinCDE system in the regulation of protein patterns and gradients.Cellular and molecular life sciences : CMLS · 2019Review
- Synthetic cell division via membrane-transforming molecular assemblies.BMC biology · 2019Review
- Tuning Protein Diffusivity with Membrane Tethers.Biochemistry · 2019Article
- In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers.Journal of visualized experiments : JoVE · 2018Article
- Reverse and forward engineering of protein pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Self-organization principles of intracellular pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- The Min-protein oscillations inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- MinE conformational switching confers robustness on self-organized Min protein patterns.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechanistic insights of the Min oscillator via cell-free reconstitution and imaging.Physical biology · 2018Review
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Authors and funding
3 authors.
Funding
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Abstract
The E. coli MinDE oscillator is a paradigm for protein self-organization and gradient formation. Previously, we reconstituted Min protein wave patterns on flat membranes as well as gradient-forming pole-to-pole oscillations in cell-shaped PDMS microcompartments. These oscillations appeared to require direct membrane interaction of the ATPase activating protein MinE. However, it remained unclear how exactly Min protein dynamics are regulated by MinE membrane binding. Here, we dissect the role of MinE's membrane targeting sequence (MTS) by reconstituting various MinE mutants in 2D and 3D geometries. We demonstrate that the MTS defines the lower limit of the concentration-dependent wavelength of Min protein patterns while restraining MinE's ability to stimulate MinD's ATPase activity. Strikingly, a markedly reduced length scale-obtainable even by single mutations-is associated with a rich variety of multistable dynamic modes in cell-shaped compartments. This dramatic remodeling in response to biochemical changes reveals a remarkable trade-off between robustness and versatility of the Min oscillator.
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