ArticleNature communications2017
Targeted two-photon chemical apoptotic ablation of defined cell types in vivo.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
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- Myelin sheaths persist for weeks following axon degeneration.Science advances · 2026Article
- Spatiotemporal dynamics of brain pericytes in ischemic stroke.Journal of neuroinflammation · 2026Review
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- Dying oligodendrocytes persist without mitochondria.bioRxiv : the preprint server for biology · 2026Article
- Mitochondria are absent from microglial processes performing surveillance, chemotaxis, and phagocytic engulfment.Nature communications · 2025Article
- Microglial cyclooxygenase-1 modulates cerebral capillary basal tone in vivo in mice.Nature communications · 2025Article
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- Mitochondrial network reorganization and transient expansion during oligodendrocyte generation.Nature communications · 2024Article
- Microglial phagocytosis of single dying oligodendrocytes is mediated by CX3CR1 but not MERTK.Cell reports · 2024Article
- TREM2 mediates MHCII-associated CD4+ T-cell response against gliomas.Neuro-oncology · 2024Article
- Molecular Basis of Neuronal and Microglial States in the Aging Brain and Impact on Cerebral Blood Vessels.International journal of molecular sciences · 2024Review
- Oligodendrocyte Maturation Alters the Cell Death Mechanisms That Cause Demyelination.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Features, Fates, and Functions of Oligodendrocyte Precursor Cells.Cold Spring Harbor perspectives in biology · 2024Review
- Longitudinal imaging of the taste bud in vivo with two-photon laser scanning microscopy.PloS one · 2024Article
- Microglial phagocytosis of single dying oligodendrocytes is mediated by CX3CR1 but not MERTK.bioRxiv : the preprint server for biology · 2023Article
- Single celliScience · 2023Article
- Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A major bottleneck limiting understanding of mechanisms and consequences of cell death in complex organisms is the inability to induce and visualize this process with spatial and temporal precision in living animals. Here we report a technique termed two-photon chemical apoptotic targeted ablation (2Phatal) that uses focal illumination with a femtosecond-pulsed laser to bleach a nucleic acid-binding dye causing dose-dependent apoptosis of individual cells without collateral damage. Using 2Phatal, we achieve precise ablation of distinct populations of neurons, glia and pericytes in the mouse brain and in zebrafish. When combined with organelle-targeted fluorescent proteins and biosensors, we uncover previously unrecognized cell-type differences in patterns of apoptosis and associated dynamics of ribosomal disassembly, calcium overload and mitochondrial fission. 2Phatal provides a powerful and rapidly adoptable platform to investigate in vivo functional consequences and neural plasticity following cell death as well as apoptosis, cell clearance and tissue remodelling in diverse organs and species.
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Registered trials
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