Evidence map›Paper›PMID 28620186›Full record

ArticleScientific reports2017

Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in Saskatchewan, Canada.

Lisanework E Ayalew, Ashish Gupta, Jenny Fricke, Khawaja Ashfaque Ahmed, Shelly Popowich, Betty Lockerbie, Suresh K Tikoo, Davor Ojkic, Susantha Gomis

Abstract read
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  3. Review
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  7. Article
  8. Orthoreovirus avis in asymptomatic broiler chickens.Tropical animal health and production · 2025
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  10. Review
  11. Article
  12. Article
  13. Article
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  16. Review
  17. Avian reovirus infection in turkeys with neurological disease in Alberta.The Canadian veterinary journal = La revue veterinaire canadienne · 2024
    Article
  18. Article
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lisanework E AyalewVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Ashish GuptaVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Jenny FrickeVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Khawaja Ashfaque AhmedVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Shelly PopowichVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Betty LockerbieVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Suresh K TikooVaccinology & Immunotherapeutic Program, School of Public Health, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.ORCID 0000-0003-4697-0179
Davor OjkicAnimal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada.
Susantha GomisVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. susantha.gomis@usask.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In recent years, emerging strains of pathogenic arthrogenic avian reovirus (ARV) have become a challenge to the chicken industry across USA and Canada causing significant economic impact. In this study, we characterized emerging variant ARV strains and examined their genetic and antigenic relationship with reference strains. We isolated 37 emerging variant ARV strains from tendons of broiler chickens with clinical cases of arthritis/tenosynovitis at commercial farms in Saskatchewan, Canada. Viral characterization using immunocytochemistry, gold-immunolabeling and electron microscopy revealed distinct features characteristic of ARV. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses of the viral Sigma C gene revealed genetic heterogeneity between the field isolates. On phylogenetic analyses, the Sigma C amino acid sequences of the isolates were clustered into four distinct genotypic groups. These ARV field strains were genetically diverse and quite distant from the vaccine and vaccine related field strains. Antibodies produced against a commercial Reo 2177

Indexed as

AnimalsArthritis, InfectiousBiopsyCanadaCattleCattle DiseasesModels, MolecularOrthoreovirus, AvianPhenotypePhylogenyPolymorphism, Restriction Fragment LengthProtein ConformationReoviridae InfectionsSeroepidemiologic StudiesSymptom AssessmentViral ProteinsViral Proteins

Identifiers

PMID28620186
PMCPMC5472580

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.