ArticleScientific reports2017
Mechanical stability of talin rod controls cell migration and substrate sensing.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis.Science advances · 2024Article
- ConFERMing the role of talin in integrin activation and mechanosignaling.Journal of cell science · 2023Article
- The mechanical cell - the role of force dependencies in synchronising protein interaction networks.Journal of cell science · 2022Review
- A primer to traction force microscopy.The Journal of biological chemistry · 2022Review
- Generic self-stabilization mechanism for biomolecular adhesions under load.Nature communications · 2022Article
- Direct observation of chaperone-modulated talin mechanics with single-molecule resolution.Communications biology · 2022Article
- Paxillin: A Hub for Mechano-Transduction from the β3 Integrin-Talin-Kindlin Axis.Frontiers in cell and developmental biology · 2022Review
- Talin in mechanotransduction and mechanomemory at a glance.Journal of cell science · 2021Article
- Protein nanomechanics in biological context.Biophysical reviews · 2021Review
- Initiation of focal adhesion assembly by talin and kindlin: A dynamic view.Protein science : a publication of the Protein Society · 2021Review
- Intrinsic Mechanical Cues and Their Impact on Stem Cells and Embryogenesis.Frontiers in cell and developmental biology · 2021Review
- Mechanoactivation of NOX2-generated ROS elicits persistent TRPM8 CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Intersection of Mechanotransduction and Regenerative Osteogenic Materials.Advanced healthcare materials · 2020Review
- Talin dissociates from RIAM and associates to vinculin sequentially in response to the actomyosin force.Nature communications · 2020Article
- Different Vinculin Binding Sites Use the Same Mechanism to Regulate Directional Force Transduction.Biophysical journal · 2020Article
- Transferrin-targeted porous silicon nanoparticles reduce glioblastoma cell migration across tight extracellular space.Scientific reports · 2020Article
- Review
- Relief of talin autoinhibition triggers a force-independent association with vinculin.The Journal of cell biology · 2020Article
- The Importance of Mechanical Forces forFrontiers in physiology · 2020Review
- Requirement for and polarized localization of integrin proteins during Drosophila wound closure.Molecular biology of the cell · 2018Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells adhere to the surrounding tissue and probe its mechanical properties by forming cell-matrix adhesions. Talin is a critical adhesion protein and participates in the transmission of mechanical signals between extracellular matrix and cell cytoskeleton. Force induced unfolding of talin rod subdomains has been proposed to act as a cellular mechanosensor, but so far evidence linking their mechanical stability and cellular response has been lacking. Here, by utilizing computationally designed mutations, we demonstrate that stepwise destabilization of the talin rod R3 subdomain decreases cellular traction force generation, which affects talin and vinculin dynamics in cell-matrix adhesions and results in the formation of talin-rich but unstable adhesions. We observed a connection between talin stability and the rate of cell migration and also found that talin destabilization affects the usage of different integrin subtypes and sensing of extracellular matrix proteins. Experiments with truncated forms of talin confirm the mechanosensory role of the talin R3 subdomain and exclude the possibility that the observed effects are caused by the release of talin head-rod autoinhibition. In conclusion, this study provides evidence into how the controlled talin rod domain unfolding acts as a key regulator of adhesion structure and function and consequently controls central cellular processes such as cell migration and substrate sensing.
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