ArticleACS nano2017
Covalent Protein Labeling and Improved Single-Molecule Optical Properties of Aqueous CdSe/CdS Quantum Dots.
Article in ACS nano, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Precise and Stable Labeling of Quantum Dots with Engineered Antibodies.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Quantum Dot Fluorescent Imaging: Using Atomic Structure Correlation Studies to Improve Photophysical Properties.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Review
- An Integrated Approach to Improve the Assay Performance of Quantum Dot-Based Lateral Flow Immunoassays by Using Silver Deposition.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2023Article
- Article
- Controlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arrays.Biochemistry · 2021Article
- X-ray-Based Techniques to Study the Nano-Bio Interface.ACS nano · 2021Article
- Quantum Dots for Improved Single-Molecule Localization Microscopy.The journal of physical chemistry. B · 2021Article
- Generation of Nanodroplet Reactors and Their Applications in In Situ Controllable Synthesis and Transportation of Ag Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices.Biomaterials science · 2020Article
- A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells.International journal of nanomedicine · 2020Article
- Engineering the S-Layer of Caulobacter crescentus as a Foundation for Stable, High-Density, 2D Living Materials.ACS synthetic biology · 2019Article
- A 25 micron-thin microscope for imaging upconverting nanoparticles with NIR-I and NIR-II illumination.Theranostics · 2019Article
- Photostable and efficient upconverting nanocrystal-based chemical sensors.Optical Materials · 2018Article
- Single Quantum Dot Tracking Illuminates Neuroscience at the Nanoscale.Chemical physics letters · 2018Article
- Article
- Iron Oxide Nanoradiomaterials: Combining Nanoscale Properties with Radioisotopes for Enhanced Molecular Imaging.Contrast media & molecular imaging · 2017Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Semiconductor quantum dots (QDs) have proven to be superior probes for single-molecule imaging compared to organic or genetically encoded fluorophores, but they are limited by difficulties in protein targeting, their larger size, and on-off blinking. Here, we report compact aqueous CdSe/CdS QDs with significantly improved bioconjugation efficiency and superior single-molecule optical properties. We have synthesized covalent protein labeling ligands (i.e., SNAP tags) that are optimized for nanoparticle use, and QDs functionalized with these ligands label SNAP-tagged proteins ∼10-fold more efficiently than existing SNAP ligands. Single-molecule analysis of these QDs shows 99% of time spent in the fluorescent on-state, ∼4-fold higher quantum efficiency than standard CdSe/ZnS QDs, and 350 million photons detected before photobleaching. Bright signals of these QDs enable us to track the stepping movement of a kinesin motor in vitro, and the improved labeling efficiency enables tracking of single kinesins in live cells.
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Registered trials
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