ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2017
Mechanisms underlying induction of allergic sensitization by Pru p 3.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- The Role of Epithelial-Derived Extracellular Vesicles in Allergic Sensitisation: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- A Human Monoclonal Antibody Displays Promiscuous Binding to Multiple Type 1 nsLTP Allergens.Journal of investigational allergology & clinical immunology · 2026Article
- The Role ofPathogens (Basel, Switzerland) · 2025Article
- Structural and Immunological Features of PR-10 Allergens: Focusing on the Major Alder Pollen Allergen Aln g 1.International journal of molecular sciences · 2024Article
- Role of Small Molecule Ligands in IgE-Mediated Allergy.Current allergy and asthma reports · 2023Review
- NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model.Scientific reports · 2022Article
- Where's the Beef? Understanding Allergic Responses to Red Meat in Alpha-Gal Syndrome.Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- The Role of Lipids in Allergic Sensitization: A Systematic Review.Frontiers in molecular biosciences · 2022Article
- Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines.Frontiers in immunology · 2022Article
- Mammalian derived lipocalin and secretoglobin respiratory allergens strongly bind ligands with potentially immune modulating properties.Frontiers in allergy · 2022Article
- How Do Pollen Allergens Sensitize?Frontiers in molecular biosciences · 2022Review
- Lipid Ligands and Allergenic LTPs: Redefining the Paradigm of the Protein-Centered Vision in Allergy.Frontiers in allergy · 2022Review
- Allergens and their associated small molecule ligands-their dual role in sensitization.Allergy · 2021Review
- Investigation of Sensitization Potential of the Soybean Allergen Gly m 4 by Using Caco-2/Immune Cells Co-Culture Model.Nutrients · 2021Article
- Non-specific lipid-transfer proteins: Allergen structure and function, cross-reactivity, sensitization, and epidemiology.Clinical and translational allergy · 2021Article
- Abundance and Stability as Common Properties of Allergens.Frontiers in allergy · 2021Review
- The Role of Sphingolipids in Allergic Disorders.Frontiers in allergy · 2021Review
- Egg yolk augments type 2 immunity by activating innate cells.European journal of nutrition · 2020Article
- Glycolipid-mediated basophil activation in alpha-gal allergy.The Journal of allergy and clinical immunology · 2020Article
- Structural Dynamics of the Lipid Antigen-Binding Site of CD1d Protein.Biomolecules · 2020Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundRecently, the nature of the lipid-ligand of Pru p 3, one of the most common plant food allergens in southern Europe, has been identified as a derivative of the alkaloid camptothecin bound to phytosphingosine. However, the origin of its immunological activity is still unknown.
objectiveWe sought to evaluate the role of the Pru p 3 lipid-ligand in the immunogenic activity of Pru p 3.
methodsIn vitro cultures of different cell types (monocyte-derived dendritic cells [moDCs], PBMCs [peripheral blood mononuclear cells] and epithelial and iNKT-hybridoma cell lines) have been used to determine the immunological capacity of the ligand, by measuring cell proliferation, maturation markers and cytokine production. To study the capacity of the lipid-ligand to promote sensitization to Pru p 3 in vivo, a mouse model of anaphylaxis to peach has been produced and changes in the humoral and basophil responses have been analysed.
resultsThe lipid-ligand of Pru p 3 induced maturation of moDCsc and proliferation of PBMCs. Its immunological activity resided in the phytosphingosine tail of the ligand. The adjuvant activity of the ligand was also confirmed in vivo, where the complex of Pru p 3-ligand induced higher levels of IgE than Pru p 3 alone. The immunological capacity of the Pru p 3 ligand was mediated by CD1d, as maturation of moDCs was inhibited by anti-CD1d antibodies and Pru p 3-ligand co-localized with CD1d on epithelial cells. Finally, Pru p 3-ligand presented by CD1d was able to interact with iNKTs. CONCLUSIONS AND CLINICAL RELEVANCE: The Pru p 3 lipid-ligand could act as an adjuvant to promote sensitization to Pru p 3, through its recognition by CD1d receptors. This intrinsic adjuvant activity of the accompanying lipid cargo could be a general essential feature of the mechanism underlying the phenomenon of allergenicity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.