Evidence map›Paper›PMID 28617577›Full record

ArticleACS chemical biology2017

Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants.

Erin Gemperline, Heidi A Horn, Kellen DeLaney, Cameron R Currie, Lingjun Li

Abstract read
In one paragraph

Article in ACS chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
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  6. Review
  7. Review
  8. Article
  9. Review
  10. In situ metabolite and lipid analysis of GluN2DAnalytical and bioanalytical chemistry · 2020
    Article
  11. Article
  12. Article
  13. Frontiers in microbiology · 2020
    Review
  14. Article
  15. Emerging evolutionary paradigms in antibiotic discovery.Journal of industrial microbiology & biotechnology · 2019
    Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Erin GemperlineDepartment of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Heidi A HornDepartment of Bacteriology, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Kellen DeLaneyDepartment of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Cameron R CurrieDepartment of Bacteriology, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
Lingjun LiDepartment of Chemistry, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.ORCID 0000-0003-0056-3869

Funding

Novel therapeutic agents from the bacterial symbionts of Brazilian invertebratesU19TW009872 · FIC · HARVARD MEDICAL SCHOOL · PI CLARDY, JON, PUPO, MONICA T · 2014 to 2018
$4.4M
Acquisition of a High-Field Dual Source FTICR-MS for Pharmaceutical ResearchS10RR029531 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI LI, LINGJUN · 2011 to 2011
$2.1M
In Situ Protein Characterization and Visualization in the Brains of Mouse Models for AutismR56MH110215 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI IKONOMIDOU, HRISSANTHI, LI, LINGJUN · 2017 to 2018
$752k
FIC NIH HHS U19 TW009872NCRR NIH HHS S10 RR029531NIMH NIH HHS R56 MH110215
6 · The paper itself

Abstract

Mass spectrometry imaging is a powerful analytical technique for detecting and determining spatial distributions of molecules within a sample. Typically, mass spectrometry imaging is limited to the analysis of thin tissue sections taken from the middle of a sample. In this work, we present a mass spectrometry imaging method for the detection of compounds produced by bacteria on the outside surface of ant exoskeletons in response to pathogen exposure. Fungus-growing ants have a specialized mutualism with Pseudonocardia, a bacterium that lives on the ants' exoskeletons and helps protect their fungal garden food source from harmful pathogens. The developed method allows for visualization of bacterial-derived compounds on the ant exoskeleton. This method demonstrates the capability to detect compounds that are specifically localized to the bacterial patch on ant exoskeletons, shows good reproducibility across individual ants, and achieves accurate mass measurements within 5 ppm error when using a high-resolution, accurate-mass mass spectrometer.

Indexed as

Mass SpectrometryActinomycetalesAnimalsAnimal ShellsAntsFungiSymbiosis

Identifiers

PMID28617577
PMCPMC6314843

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.