ArticleScientific reports2017
Serotyping dengue virus with isothermal amplification and a portable sequencer.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Pooled it
- Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia.Diagnostics (Basel, Switzerland) · 2025Article
- Multiplexing LAMP Assays: A Methodological Review and Diagnostic Application.International journal of molecular sciences · 2024Review
- Arbovirus surveillance in mosquitoes: Historical methods, emerging technologies, and challenges ahead.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Analytical and diagnostic performance characteristics of reverse-transcriptase loop-mediated isothermal amplification assays for dengue virus serotypes 1-4: A scoping review to inform potential use in portable molecular diagnostic devices.PLOS global public health · 2023Article
- Rapid, in-field deployable, avian influenza virus haemagglutinin characterisation tool using MinION technology.Scientific reports · 2022Article
- Article
- Global research alliance in infectious disease: a collaborative effort to combat infectious diseases through dissemination of portable sequencing.BMC research notes · 2022Article
- Systematic Comparison of Nanopore and Illumina Sequencing for the Detection of Plant Viruses and Viroids Using Total RNA Sequencing Approach.Frontiers in microbiology · 2022Article
- Dengue Detection: Advances in Diagnostic Tools from Conventional Technology to Point of Care.Biosensors · 2021Review
- Nanopore Sequencing Is a Credible Alternative to Recover Complete Genomes of Geminiviruses.Microorganisms · 2021Article
- Characterization of Emerging Swine Viral Diseases through Oxford Nanopore Sequencing Using Senecavirus A as a Model.Viruses · 2020Article
- Rapid and economical drug resistance profiling with Nanopore MinION for clinical specimens with low bacillary burden of Mycobacterium tuberculosis.BMC research notes · 2020Article
- Molecular Approaches for Low-Cost Point-of-Care Pathogen Detection in Agriculture and Forestry.Frontiers in plant science · 2020Review
- Portable sequencer in the fight against infectious disease.Journal of human genetics · 2020Review
- Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases.Parasite (Paris, France) · 2020Article
- Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies.Scientific data · 2019Article
- Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing.PLoS neglected tropical diseases · 2019Article
- Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria.Sensors (Basel, Switzerland) · 2019Review
- Nanopore-based detection and characterization of yam viruses.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
15 authors at 10 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent development of a nanopore-type portable DNA sequencer has changed the way we think about DNA sequencing. We can perform sequencing directly in the field, where we collect the samples. Here, we report the development of a novel method to detect and genotype tropical disease pathogens, using dengue fever as a model. By combining the sequencer with isothermal amplification that only requires a water bath, we were able to amplify and sequence target viral genomes with ease. Starting from a serum sample, the entire procedure could be finished in a single day. The analysis of blood samples collected from 141 Indonesian patients demonstrated that this method enables the clinical identification and serotyping of the dengue virus with high sensitivity and specificity. The overall successful detection rate was 79%, and a total of 58 SNVs were detected. Similar analyses were conducted on 80 Vietnamese and 12 Thai samples with similar performance. Based on the obtained sequence information, we demonstrated that this approach is able to produce indispensable information for etiologically analyzing annual or regional diversifications of the pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.