ArticleScientific reports2017
Bone morphogenetic protein 4 promotes the survival and preserves the structure of flow-sorted Bhlhb5+ cochlear spiral ganglion neurons in vitro.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Internal Acoustic Meatus: Morphological Peculiarities and Clinical Implications.Audiology research · 2026Article
- Shikonin Attenuates Cochlear Spiral Ganglion Neuron Degeneration by Activating Nrf2-ARE Signaling Pathway.Frontiers in molecular neuroscience · 2022Article
- Key Signaling Pathways Regulate the Development and Survival of Auditory Hair Cells.Neural plasticity · 2021Review
- Tlr2/4 Double Knockout Attenuates the Degeneration of Primary Auditory Neurons: Potential Mechanisms From Transcriptomic Perspectives.Frontiers in cell and developmental biology · 2021Article
- Syndromic Deafness GeneFrontiers in cell and developmental biology · 2021Article
- The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis.Frontiers in bioengineering and biotechnology · 2021Article
- Promising Applications of Nanoparticles in the Treatment of Hearing Loss.Frontiers in cell and developmental biology · 2021Review
- Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion NeuronsNeural plasticity · 2020Article
- Hair cell regeneration from inner ear progenitors in the mammalian cochlea.American journal of stem cells · 2020Review
- A Neurophysiological Study of Musical Pitch Identification in Mandarin-Speaking Cochlear Implant Users.Neural plasticity · 2020Article
- Blebbistatin Inhibits Neomycin-Induced Apoptosis in Hair Cell-Like HEI-OC-1 Cells and in Cochlear Hair Cells.Frontiers in cellular neuroscience · 2019Article
- Pre-treatment With Fasudil Prevents Neomycin-Induced Hair Cell Damage by Reducing the Accumulation of Reactive Oxygen Species.Frontiers in molecular neuroscience · 2019Article
- Novel compounds protect auditory hair cells against gentamycin-induced apoptosis by maintaining the expression level of H3K4me2.Drug delivery · 2018Article
- The Characteristic and Short-Term Prognosis of Tinnitus Associated with Sudden Sensorineural Hearing Loss.Neural plasticity · 2018Article
- Postnatal Development of Microglia-Like Cells in Mouse Cochlea.Neural plasticity · 2018Article
- Bone morphogenetic proteins and inner ear development.Journal of Zhejiang University. Science. BReview
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SGNs are the primary auditory neurons, and damage or loss of SGNs leads to sensorineural hearing loss. BMP4 is a growth factor that belongs to the TGF-β superfamily and has been shown to play a key role during development, but little is known about its effect on postnatal cochlear SGNs in mice. In this study, we used the P3 Bhlhb5-cre/tdTomato transgenic mouse model and FACS to isolate a pure population of Bhlhb5+ SGNs. We found that BMP4 significantly promoted SGN survival after 7 days of culture. We observed fewer apoptotic cells and decreased expression of pro-apoptotic marker genes after BMP4 treatment. We also found that BMP4 promoted monopolar neurite outgrowth of isolated SGNs, and high concentrations of BMP4 preserved the number and the length of neurites in the explant culture of the modiolus harboring the SGNs. We showed that high concentration of BMP4 enhanced neurite growth as determined by the higher average number of filopodia and the larger area of the growth cone. Finally, we found that high concentrations of BMP4 significantly elevated the synapse density of SGNs in explant culture. Thus, our findings suggest that BMP4 has the potential to promote the survival and preserve the structure of SGNs.
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