ArticleBMC genomics2017
Integrated metabolome and transcriptome analysis of Magnolia champaca identifies biosynthetic pathways for floral volatile organic compounds.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Effects of drying methods on the functional components and volatile organic compounds of Atractylodes chinensis.BMC plant biology · 2026Article
- Combined Metabolome and Transcriptome Analysis of Floral Organ Development inPlants (Basel, Switzerland) · 2026Article
- Floral scent profiling and candidate gene discovery inFrontiers in plant science · 2026Article
- Multi-Omics Analysis Decodes Biosynthesis of Specialized Metabolites Constituting the Therapeutic Terrains ofInternational journal of molecular sciences · 2025Article
- Transcriptomic Analysis Reveals Adaptive Evolution and Conservation Implications for the EndangeredGenes · 2024Article
- Physiological and Biochemical Analysis Revealing the Key Factors Influencing 2-Phenylethanol and Benzyl Alcohol Production in Crabapple Flowers.Plants (Basel, Switzerland) · 2024Article
- Transcriptome and volatile compounds analyses of floral development provide insight into floral scent formation inFrontiers in plant science · 2024Article
- A matrix-centered view of mass spectrometry platform innovation for volatilome research.Frontiers in molecular biosciences · 2024Review
- Molecular mechanisms underlying floral fragrance inFrontiers in plant science · 2024Article
- Identification of Floral Volatile Components and Expression Analysis of Controlling Gene inPlants (Basel, Switzerland) · 2023Article
- Transcriptomic and metabolomic data reveal key genes that are involved in the phenylpropanoid pathway and regulate the floral fragrance of Rhododendron fortunei.BMC plant biology · 2023Article
- Volatile metabolome and floral transcriptome analyses reveal the volatile components of strongly fragrant progeny ofFrontiers in plant science · 2023Article
- Transcriptomic analysis of deceptively pollinated Arum maculatum (Araceae) reveals association between terpene synthase expression in floral trap chamber and species-specific pollinator attraction.G3 (Bethesda, Md.) · 2022Article
- Comparative Transcriptome Analysis Reveals Coordinated Transcriptional Regulation of Central and Secondary Metabolism in the Trichomes of Cannabis Cultivars.International journal of molecular sciences · 2022Article
- Comprehensive Flavor Analysis of Volatile Components During the Vase Period of Cut Lily (Frontiers in plant science · 2022Article
- The chromosome-scale genome ofiScience · 2021Article
- Identification of VOCs in essential oils extracted using ultrasound- and microwave-assisted methods from sweet cherry flower.Scientific reports · 2021Article
- Integrated Analysis of the Transcriptome and Metabolome Reveals Genes Involved in Terpenoid and Flavonoid Biosynthesis in the Loblolly Pine (Frontiers in plant science · 2021Article
- Integration of Transcriptome and Methylome Analyses Provides Insight Into the Pathway of Floral Scent Biosynthesis inFrontiers in genetics · 2021Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMagnolia champaca, commonly known as champak is a well-known tree due to its highly fragrant flowers. Champak floral scent is attributed to a complex mix of volatile organic compounds (VOCs). These aromatic flowers are widely used in flavors and fragrances industry. Despite its commercial importance, the VOC biosynthesis pathways in these flowers are largely unknown. Here, we combine metabolite and RNA sequencing (RNA-seq) analyses of fully opened champak flowers to discover the active VOC biosynthesis pathways as well as floral scent-related genes.
resultsVolatile collection by headspace method and analysis by gas chromatography-mass spectrometry (GC-MS) identified a total of 43 VOCs from fully opened champak flowers, of which 46.9% were terpenoids, 38.9% were volatile esters and 5.2% belonged to phenylpropanoids/benzenoids. Sequencing and de novo assembly of champak flower transcriptome yielded 47,688 non-redundant unigenes. Transcriptome assembly was validated using standard polymerase chain reaction (PCR) based approach for randomly selected unigenes. The detailed profiles of VOCs led to the discovery of pathways and genes involved in floral scent biosynthesis from RNA-seq data. Analysis of expression levels of many floral-scent biosynthesis-related unigenes in flowers and leaves showed that most of them were expressed higher in flowers than in leaf tissues. Moreover, our metabolite-guided transcriptomics, in vitro and in vivo enzyme assays and transgenic studies identified (R)-linalool synthase that is essential for the production of major VOCs of champak flowers, (R)-linalool and linalool oxides.
conclusionAs our study is the first report on transcriptome analysis of Magnolia champaca, this transcriptome dataset that serves as an important public information for functional genomics will not only facilitate better understanding of ecological functions of champak floral VOCs, but also provide biotechnological targets for sustainable production of champak floral scent.
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