ArticleScientific reports2017
A microengineered model of RBC transfusion-induced pulmonary vascular injury.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- A multicellular vessel-on-a-chip model reveals context-dependent roles for platelets in inflammation and inflammatory hemostasis.Blood vessels, thrombosis & hemostasis · 2024Article
- Advances in Microfluidics for Single Red Blood Cell Analysis.Biosensors · 2023Review
- Lung-on-a-Chip Models of the Lung Parenchyma.Advances in experimental medicine and biology · 2023Article
- Microfluidic models of the human circulatory system: versatile platforms for exploring mechanobiology and disease modeling.Biophysical reviews · 2021Review
- Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques.Scientific reports · 2021Article
- Microvascularized tumor organoids-on-chips: advancing preclinical drug screening with pathophysiological relevance.Nano convergence · 2021Review
- Collateral damage: necroptosis in the development of lung injury.American journal of physiology. Lung cellular and molecular physiology · 2020Review
- Vascularized Microfluidics and the Blood-Endothelium Interface.Micromachines · 2019Review
- Endothelialized flow models for blood transfusion research.Haematologica · 2019Article
- Stem cell-based Lung-on-Chips: The best of both worlds?Advanced drug delivery reviews · 2019Review
- A bioinspired microfluidic model of liquid plug-induced mechanical airway injury.Biomicrofluidics · 2018Article
- Engineering "Endothelialized" Microfluidics for Investigating Vascular and Hematologic Processes Using Non-Traditional Fabrication Techniques.Current opinion in biomedical engineering · 2018Article
- The new frontiers of the targeted interventions in the pulmonary vasculature: precision and safety (2017 Grover Conference Series).Pulmonary circulationArticle
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Red blood cell (RBC) transfusion poses significant risks to critically ill patients by increasing their susceptibility to acute respiratory distress syndrome. While the underlying mechanisms of this life-threatening syndrome remain elusive, studies suggest that RBC-induced microvascular injury in the distal lung plays a central role in the development of lung injury following blood transfusion. Here we present a novel microengineering strategy to model and investigate this key disease process. Specifically, we created a microdevice for culturing primary human lung endothelial cells under physiological flow conditions to recapitulate the morphology and hemodynamic environment of the pulmonary microvascular endothelium in vivo. Perfusion of the microengineered vessel with human RBCs resulted in abnormal cytoskeletal rearrangement and release of intracellular molecules associated with regulated necrotic cell death, replicating the characteristics of acute endothelial injury in transfused lungs in vivo. Our data also revealed the significant effect of hemodynamic shear stress on RBC-induced microvascular injury. Furthermore, we integrated the microfluidic endothelium with a computer-controlled mechanical stretching system to show that breathing-induced physiological deformation of the pulmonary microvasculature may exacerbate vascular injury during RBC transfusion. Our biomimetic microsystem provides an enabling platform to mechanistically study transfusion-associated pulmonary vascular complications in susceptible patient populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.