ArticleFuture microbiology2017
Nf-GH, a glycosidase secreted by Naegleria fowleri, causes mucin degradation: an in vitro and in vivo study.
Article in Future microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Virulence ofbioRxiv : the preprint server for biology · 2025Article
- Eating the brain - A multidisciplinary study provides new insights into the mechanisms underlying the cytopathogenicity of Naegleria fowleri.PLoS pathogens · 2025Article
- A review of the mechanism, diagnosis, and treatment ofFrontiers in microbiology · 2025Review
- Identification, Biochemical Characterization, and In Vivo Detection of a Zn-Metalloprotease with Collagenase Activity fromInternational journal of molecular sciences · 2024Article
- Lactoferrin and Lysozyme Inhibit the Proteolytic Activity and Cytopathic Effect ofPathogens (Basel, Switzerland) · 2024Article
- Role of cathepsin B of Naegleria fowleri during primary amebic meningoencephalitis.Parasitology research · 2022Article
- Activity of Apo-Lactoferrin on Pathogenic Protozoa.Pharmaceutics · 2022Review
- Article
- Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri.BMC biology · 2021Article
- Nanopore sequencing improves the draft genome of the human pathogenic amoeba Naegleria fowleri.Scientific reports · 2019Article
- Enzymatic and Structural Characterization of theAntimicrobial agents and chemotherapy · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
aimThe aim of this work was to identify, characterize and evaluate the pathogenic role of mucinolytic activity released by Naegleria fowleri. MATERIALS &
methodsZymograms, protease inhibitors, anion exchange chromatography, MALDI-TOF-MS, enzymatic assays, Western blot, and confocal microscopy were used to identify and characterize a secreted mucinase; inhibition assays using antibodies, dot-blots and mouse survival tests were used to evaluate the mucinase as a virulence factor.
resultsA 94-kDa protein with mucinolytic activity was inducible and abolished by p-hydroxymercuribenzoate. MALDI-TOF-MS identified a glycoside hydrolase. Specific antibodies against N. fowleri-glycoside hydrolase inhibit cellular damage and MUC5AC degradation, and delay mouse mortality.
conclusionOur findings suggest that secretory products from N. fowleri play an important role in mucus degradation during the invasion process.
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Registered trials
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