Evidence map›Paper›PMID 28608322›Full record

ArticleTransgenic research2017

Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production.

F Kent Hamra, Christopher T Richie, Brandon K Harvey

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transgenic research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Rattus norvegicus Spermatogenesis Colony-Forming Assays.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

F Kent HamraDepartment of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX, 75390, USA. kent.hamra@utsouthwestern.edu.
Christopher T RichieNational Institute on Drug Abuse, National Institutes of Health, 251 Bayview Blvd, Baltimore, MD, 21224, USA.
Brandon K HarveyNational Institute on Drug Abuse, National Institutes of Health, 251 Bayview Blvd, Baltimore, MD, 21224, USA.
National Institute on Drug Abuse · USNational Institutes of Health · USThe University of Texas Southwestern Medical Center · US

Funding

Cellular mechanisms of neuronal dysfunction in addiction and neurodegenerationZIADA000618 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI HARVEY, BRANDON · 2017 to 2025
$12.9M
Optogenetics and Transgenic Technology CoreZICDA000555 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI HARVEY, BRANDON · 2009 to 2017
$10.0M
Gene Delivery and AddictionZ01DA000526 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI HARVEY, BRANDON · 2008 to 2008
$498k
Intramural NIH HHS Z01 DA000526Intramural NIH HHS ZIC DA000555
6 · The paper itself

Abstract

Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in donor spermatogonial stem cells. Long Evans rat spermatogonial lines were derived from freshly isolated laminin-binding spermatogonia. Laminin-binding spermatogonia were cultured over multiple passages on fibroblast feeder layers in serum-free culture medium containing GDNF and FGF2. Long Evans rat spermatogonial lines were genetically modified by transposon transduction to express a germline, tdTomato reporter gene. Donor rat spermatogonial lines robustly regenerated spermatogenesis after transplantation into testes of busulfan-treated, allogenic, Long Evans rats. Donor-derived spermatogenesis largely restored testis size in the chemically sterilized, recipient Long Evans rats. Recipient Long Evans rats stably transmitted the tdTomato germline marker to subsequent generations. Overall, Long Evans rat spermatogonial lines provided effective donor germline vectors for genetically modifying Long Evans rats.

Indexed as

AnimalsDNA Transposable ElementsGenes, ReporterGerm CellsLamininMaleRatsRats, Long-EvansRats, TransgenicSolanum lycopersicumSpermatogenesisSpermatogoniaStem CellsTestisDNA Transposable ElementsLamininAllogeneic transplantationDrug abuseDrug addictionGermline editingSpermatogonial stem cellsTransgenic rats

Identifiers

PMID28608322
PMCPMC7336504
OpenAlexW2625158635

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.