ArticleMethods in molecular biology (Clifton, N.J.)2017
Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors.
Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 75 citations in OpenAlex.
- Assessing Sarcocornia as a Salt Substitute: Effects on Lipid Profile and Gelatinase Activity.Nutrients · 2024Trial
- Anxiolytic and antidepressant effects of astaxanthin: behavioral and mechanistic insights in a rat model.Frontiers in pharmacology · 2026Article
- Clinical impacts of Artocarpus lakoocha agglutinin-binding glycans for prognosis and treatment of cholangiocarcinoma.Scientific reports · 2025Article
- Zymography: A Simple and Powerful Tool for the Assessment of MMP-2 and MMP-9 in Pathological Conditions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Assessing MMP-2/9 Proteolytic Activity and Activation Status by Zymography in Preclinical and Clinical Tissue Samples.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Analysis of Metalloproteinase Secretion in Cancer Cells by Zymography.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Betanin-encapsulated starch nanoparticles: synthesis and cytotoxic effect on colon cancer.3 Biotech · 2024Article
- Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples.BMC biotechnology · 2024Article
- The Role of Macrophage Inhibitory Factor in TAA-Induced Liver Fibrosis in Mice: Modulatory Effects of Betaine.Biomedicines · 2024Article
- Deflamin Attenuated Lung Tissue Damage in an Ozone-Induced COPD Murine Model by Regulating MMP-9 Catalytic Activity.International journal of molecular sciences · 2024Article
- Implications of the Matrix Metalloproteinases, Their Tissue Inhibitors and Some Other Inflammatory Mediators Expression Levels in Children Obesity-Related Phenotypes.Journal of personalized medicine · 2024Article
- Regulation of MMP-2 by IL-8 in Vascular Endothelial Cells: Probable Mechanism for Endothelial Dysfunction in Women with Preeclampsia.International journal of molecular sciences · 2023Article
- TheFoods (Basel, Switzerland) · 2023Article
- Sodium Nitrite Attenuates Reduced Activity of Vascular Matrix Metalloproteinase-2 and Vascular Hyper-Reactivity and Increased Systolic Blood Pressure Induced by the Placental Ischemia Model of Preeclampsia in Anesthetized Rats.International journal of molecular sciences · 2023Article
- Resistance to Degradation of Silk Fibroin Hydrogels Exposed to Neuroinflammatory Environments.Polymers · 2023Article
- Characterization of Active MMP9 in Chronic Inflammatory Diseases Using a Novel Anti-MMP9 Antibody.Antibodies (Basel, Switzerland) · 2023Article
- Enzymes of Fibrosis in Chronic Liver Disease.Biomedicines · 2022Article
- Differential expression of aqueous humor microRNAs in central retinal vein occlusion and its association with matrix metalloproteinases: a pilot study.Scientific reports · 2022Article
- In Vitro Models to Study Angiogenesis and Vasculature.Methods in molecular biology (Clifton, N.J.) · 2022Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Matrix metalloproteinases (MMPs) are proteolytic enzymes that degrade various components of the extracellular matrix (ECM) and play a role in tissue remodeling. Changes in MMPs have been observed in cancer, connective tissue disorders, and vascular disease, and both endogenous tissue inhibitors of MMPs (TIMPs) and synthetic MMP inhibitors (MMPIs) have been evaluated as modulators of MMP activity in various biological systems. Zymography is a simple technique that is commonly used to assess MMP activity and the efficacy of MMPIs. Also, reverse zymography is a modified technique to study the activity of endogenous TIMPs. However, problems are often encountered during the zymography procedure, which could interfere with accurate assessment of MMP activity in control specimens, and thus make it difficult to determine the pathological changes in MMPs and their responsiveness to MMPIs. Simplified protocols for preparation of experimental solutions, tissue preparation, regular and reverse zymography procedures, and zymogram analysis are presented. Additional helpful tips to troubleshoot problems in the zymography technique and to enhance the quality of the zymograms should make it more feasible to determine the changes in MMPs and assess the efficacy of MMPIs in modulating MMP activity in various biological systems and pathological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.